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Literature summary for 1.1.1.149 extracted from

  • Ma, H.; Penning, T.M.
    Conversion of mammalian 3alpha-hydroxysteroid dehydrogenase to 20alpha-hydroxysteroid dehydrogenase using loop chimeras: changing specificity from androgens to progestins (1999), Proc. Natl. Acad. Sci. USA, 96, 11161-11166.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.149

Cloned(Commentary)

Cloned (Comment) Organism
subcloned, overexpressed in Escherichia coli DH5alpha or C41 (DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information loops of 3alpha-hydroxysteroid dehydrogenase exchanged by PCR generate 20alpha-hydroxysteroid dehydrogenase, loops A, B and C yields total 20alpha-hydroxysteroid dehydrogenase activity, replacement of A and C yields bifunctional enzyme, several point mutations in the pocket of 3alpha-hydroxysteroid dehydrogenase show no 20alpha-hydroxysteroid dehydrogenase activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 20alpha-hydroxyprogesterone
-
 Rattus norvegicus
0.12
-
 20alpha-hydroxyprogesterone A, B and C loop changed, total converted enzyme Rattus norvegicus
0.13
-
 20alpha-hydroxyprogesterone A and C loop changed, bifunctional enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange and affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20alpha-hydroxyprogesterone + NADP+
-
 Rattus norvegicus progesterone + NADPH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 20alpha-hydroxyprogesterone +/-0.4, A, B and C loop changed, totally converted enzyme Rattus norvegicus
0.055
-
 20alpha-hydroxyprogesterone +/-0.4, A and C loop changed, bifunctional enzyme Rattus norvegicus
1.06
-
 20alpha-hydroxyprogesterone +/-0.3 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus