Crystallization (Comment) | Organism |
---|---|
- |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
17-hydroxyprogesterone | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Bos taurus |
40000 | - |
SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, affinity chromatography, gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.115 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, in presence of glycerol and DTT | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NAD(P)H + H+ | NADPH preferred | Bos taurus | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+ | - |
? | |
additional information | androstenedione and corticosterone not substrates | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000 SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable in glycerol and dithiothreitol | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
steroid reduction | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bos taurus | |
NADPH | preferred | Bos taurus |