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Literature summary for 1.1.1.149 extracted from
- Purification of rat ovary 20alpha-hydroxysteroid dehydrogenase by affinity chromatography (1979), J. Steroid Biochem., 11, 1443-1449.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
young, pregnant, female | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.318 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, cytosol preparation prior to purification, several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 20alpha-hydroxyprogesterone + NADP+ | - |
Rattus norvegicus | progesterone + NADPH | - |
? |  |
| additional information | no activity with 20beta-hydroxypregn-4-en-3-one, 5alpha-pregnan-3,20-dione, 3beta-hydroxy-5alpha-pregnan-20-one and 3alpha,20alpha-dihydroxy-5alpha-pregnane | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
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