Cloned (Comment) | Organism |
---|---|
cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q91WR5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | high expression level | Mus musculus | - |
additional information | enzyme tissue distribution in male and female mice, overview | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH | - |
Mus musculus | 5alpha-androstane-3alpha,17alpha-diol + NADP+ | - |
? | |
5alpha-pregnane-3,20-dione + NADPH + H+ | - |
Mus musculus | 3alpha,5alpha-3-hydroxypregnan-20-one + NADP+ | - |
? | |
androstanedione + NADPH | - |
Mus musculus | 5alpha-androstane-3-one-17alpha-ol + NADP+ | - |
? | |
androstenedione + NADPH + H+ | - |
Mus musculus | epitestosterone + NADP+ | - |
? | |
androsterone + NADPH | - |
Mus musculus | 5alpha-androstane-3alpha,17alpha-diol + NADP+ | - |
? | |
dehydroepiandrosterone + NADPH | - |
Mus musculus | 5-androstene-3alpha,17alpha-diol + NADP+ | - |
? | |
additional information | the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone | Mus musculus | ? | - |
? | |
additional information | substrate specificity of the recombinant enzyme, overview | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17alpha-HSD | - |
Mus musculus |
17alpha-hydroxysteroid dehydrogenase | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |