Cloned (Comment) | Organism |
---|---|
cloning from clone 5193867, ATCC clone 7277078, expression of isozyme 11beta-HSD1 catalytic domain mutant C272S, comprising residues 24-292 proceeded by a 17-amino acid-affinity tag, in Escherichia coli strain DH10b-Tir | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant isozyme 11beta-HSD1 catalytic domain mutant C272S, the nanovolume crystallization method, the reservoir solution contains 20% PEG 3350, and 0.1 M MES, pH 6.2, soaking of crystals in 1 mM lutetium acetate reservoir solution, two crystal forms, X-ray diffraction structure determination and analysis at 1.55-2.45 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | oxidation of the enzymes disulfides with K3Fe(CN)6 reduces the activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 | Homo sapiens | |
0.042 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 oxidized with K3Fe(CN)6 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant isozyme 11beta-HSD1 catalytic domain mutant C272S from Escherichia coli strain DH10b-Tir by affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisol + NADP+ | isozyme 11beta-HSD1, steroid binding structure, overview | Homo sapiens | cortisone + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | subunit structure and assembly, quarternary structure with interface-closed or interface-opened conformations, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 | Homo sapiens | |
0.078 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 oxidized with K3Fe(CN)6 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |