Literature summary for 1.1.1.146 extracted from

Shafqat, N.; Elleby, B.; Svensson, S.; Shafqat, J.; Jornvall, H.; Abrahmsen, L.; Oppermann, U.
Comparative enzymology of 11beta -hydroxysteroid dehydrogenase type 1 from glucocorticoid resistant (guinea pig) versus sensitive (human) species (2003), J. Biol. Chem., 278, 2030-2035.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli	Cavia porcellus
expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00028	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1	Cavia porcellus
0.0003	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1	Homo sapiens
0.0006	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Cavia porcellus
0.0008	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Homo sapiens
0.002	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris	Cavia porcellus
0.002	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris	Homo sapiens
0.003	-	cortisol	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Cavia porcellus
0.003	-	cortisol	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Homo sapiens
0.008	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1	Cavia porcellus
0.009	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cortisone + NADPH + H+	Cavia porcellus	-	cortisol + NADP+	-	r
cortisone + NADPH + H+	Homo sapiens	-	cortisol + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Cavia porcellus	-	-	-
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged-11beta-HSD1 expressed in Escherichia coli	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	subcapsular areas of adrenal gland	Cavia porcellus	-
liver	central vein	Cavia porcellus	-
liver	central vein	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cortisone + NADPH + H+	-	Cavia porcellus	cortisol + NADP+	-	r
cortisone + NADPH + H+	-	Homo sapiens	cortisol + NADP+	-	r

Synonyms

Synonyms	Comment	Organism
11beta-HSD1	-	Cavia porcellus
11beta-HSD1	-	Homo sapiens
11beta-hydroxysteroid dehydrogenase type 1	-	Cavia porcellus
11beta-hydroxysteroid dehydrogenase type 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	cortisol	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Cavia porcellus
0.19	-	cortisol	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Homo sapiens
0.3	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Cavia porcellus
0.4	-	cortisone	37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli	Homo sapiens

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Release 2023.1 (January 2023)