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Literature summary for 1.1.1.146 extracted from

  • Hundertmark, S.; Ragosch, V.; Schein, B.; Buhler, H.; Fromm, M.; Lorenz, U.; Weitzel, H.K.
    11-beta-Hydroxysteroid dehydrogenase of rat lung: Enzyme kinetic, oxidase-reductase ration, electrolyte and trace element dependence (1993), Enzyme Protein, 47, 83-91.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADP+ 50% inhibition at 220 nmol Rattus norvegicus
NADP+ 50% inhibition at 220 nmol Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00108
-
11beta,21-dihydroxypregn-4-en-3,20-dione
-
Rattus sp.
0.00108
-
11beta,21-dihydroxypregn-4-en-3,20-dione i.e. cortisol,temperature not specified in the publication, pH 9 Rattus norvegicus
0.00156
-
21-hydroxy-pregn-4-en-3,11,20-trione
-
Rattus sp.
0.00156
-
21-hydroxy-pregn-4-en-3,11,20-trione temperature not specified in the publication, pH 9 Rattus norvegicus
0.00372
-
NADPH
-
Rattus sp.
0.00372
-
NADPH temperature not specified in the publication, pH 9 Rattus norvegicus
0.00427
-
NADP+
-
Rattus sp.
0.00427
-
NADP+ temperature not specified in the publication, pH 9 Rattus norvegicus
0.00875
-
17,21-dihydroxy-pregn-4-ene-3,11,20-trione i.e. cortisone Rattus sp.
0.00875
-
17,21-dihydroxy-pregn-4-ene-3,11,20-trione i.e. cortisone, temperature not specified in the publication, pH 9 Rattus norvegicus
0.0249
-
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione i.e. cortisol Rattus sp.
0.091
-
NAD+
-
Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ Rattus sp. no enzyme activity in reverse reaction direction 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+
-
ir
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ Rattus sp. i.e. corticosterone 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+
-
ir
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ Rattus sp. 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
r
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ Rattus norvegicus i.e. corticosterone, 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH
-
r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH Rattus sp. 5.5 times lower affinity than for 11-dehydrocorticosterone 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH Rattus sp. i.e. cortisone 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH Rattus norvegicus i.e. cortisone, 5.5 times lower affinity than for 11-dehydrocorticosterone 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lung fetal, adult Rattus norvegicus
-
lung fetal, adult Rattus sp.
-

Storage Stability

Storage Stability Organism
-20°C, 0.01 M sodium phosphate, pH 7.4, 0.25 M sucrose, 6 weeks Rattus norvegicus
-20°C, 0.01 M sodium phosphate, pH 7.4, 0.25 M sucrose, 6 weeks Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ no enzyme activity in reverse reaction direction Rattus sp. 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+
-
ir
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ i.e. corticosterone Rattus sp. 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+
-
ir
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity Rattus sp. 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
r
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ i.e. corticosterone, 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity Rattus norvegicus 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH
-
r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH 5.5 times lower affinity than for 11-dehydrocorticosterone Rattus sp. 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone Rattus sp. 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone, 5.5 times lower affinity than for 11-dehydrocorticosterone Rattus norvegicus 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reductase activity Rattus norvegicus
6.5
-
reductase activity Rattus sp.
9
-
oxidase activity Rattus norvegicus
9
-
oxidase activity Rattus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ 20 times lower affinity than for NADP+ Rattus sp.
NADH no substrate affinity Rattus sp.
NADP+
-
Rattus sp.
NADP+ 20 times higher affinity than for NAD+ Rattus norvegicus
NADPH
-
Rattus sp.