Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | 50% inhibition at 220 nmol | Rattus norvegicus | |
NADP+ | 50% inhibition at 220 nmol | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00108 | - |
11beta,21-dihydroxypregn-4-en-3,20-dione | - |
Rattus sp. | |
0.00108 | - |
11beta,21-dihydroxypregn-4-en-3,20-dione | i.e. cortisol,temperature not specified in the publication, pH 9 | Rattus norvegicus | |
0.00156 | - |
21-hydroxy-pregn-4-en-3,11,20-trione | - |
Rattus sp. | |
0.00156 | - |
21-hydroxy-pregn-4-en-3,11,20-trione | temperature not specified in the publication, pH 9 | Rattus norvegicus | |
0.00372 | - |
NADPH | - |
Rattus sp. | |
0.00372 | - |
NADPH | temperature not specified in the publication, pH 9 | Rattus norvegicus | |
0.00427 | - |
NADP+ | - |
Rattus sp. | |
0.00427 | - |
NADP+ | temperature not specified in the publication, pH 9 | Rattus norvegicus | |
0.00875 | - |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione | i.e. cortisone | Rattus sp. | |
0.00875 | - |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione | i.e. cortisone, temperature not specified in the publication, pH 9 | Rattus norvegicus | |
0.0249 | - |
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione | i.e. cortisol | Rattus sp. | |
0.091 | - |
NAD+ | - |
Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ | Rattus sp. | no enzyme activity in reverse reaction direction | 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+ | - |
ir | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ | Rattus sp. | i.e. corticosterone | 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+ | - |
ir | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | Rattus sp. | 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+ | - |
r | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | Rattus norvegicus | i.e. corticosterone, 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH | - |
r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Rattus sp. | 5.5 times lower affinity than for 11-dehydrocorticosterone | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Rattus sp. | i.e. cortisone | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Rattus norvegicus | i.e. cortisone, 5.5 times lower affinity than for 11-dehydrocorticosterone | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | fetal, adult | Rattus norvegicus | - |
lung | fetal, adult | Rattus sp. | - |
Storage Stability | Organism |
---|---|
-20°C, 0.01 M sodium phosphate, pH 7.4, 0.25 M sucrose, 6 weeks | Rattus norvegicus |
-20°C, 0.01 M sodium phosphate, pH 7.4, 0.25 M sucrose, 6 weeks | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ | no enzyme activity in reverse reaction direction | Rattus sp. | 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+ | - |
ir | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NAD+ | i.e. corticosterone | Rattus sp. | 21-hydroxy-pregn-4-en-3,11,20-trione + NADH + H+ | - |
ir | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity | Rattus sp. | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+ | - |
r | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | i.e. corticosterone, 5.5times higher affinity for 11-dehydrocorticosterone than for cortisone, 24times higher affinity of corticosterone to 11-dehydrogenase than of cortisol, predominant reductive activity | Rattus norvegicus | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH | - |
r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | 5.5 times lower affinity than for 11-dehydrocorticosterone | Rattus sp. | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | Rattus sp. | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone, 5.5 times lower affinity than for 11-dehydrocorticosterone | Rattus norvegicus | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reductase activity | Rattus norvegicus |
6.5 | - |
reductase activity | Rattus sp. |
9 | - |
oxidase activity | Rattus norvegicus |
9 | - |
oxidase activity | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 20 times lower affinity than for NADP+ | Rattus sp. | |
NADH | no substrate affinity | Rattus sp. | |
NADP+ | - |
Rattus sp. | |
NADP+ | 20 times higher affinity than for NAD+ | Rattus norvegicus | |
NADPH | - |
Rattus sp. |