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Literature summary for 1.1.1.145 extracted from

  • Rabe, T.; Brandstetter, K.; Kellermann, J.; Runnebaum, B.
    Partial characterization of placental 3beta-hydroxysteroid dehydrogenase (EC 1.1.1.145), delta 4-5isomerase (EC 5.3.3.1) in human term placental mitochondria (1982), J. Steroid Biochem., 17, 427-433.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM: strong Homo sapiens
Co2+ 1 mM: weak, 100 mM: strong Homo sapiens
Cu2+ 1 mM: strong Homo sapiens
Fe2+ 1 mM: weak, 100 mM: strong Homo sapiens
Mn2+ 1 mM: weak Homo sapiens
Ni2+ 1 mM: weak, 100 mM: strong Homo sapiens
Zn2+ 1 mM: strong Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00007
-
pregnenolone
-
Homo sapiens
0.02
-
NAD+
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect of Ca2+, Sr2+, Ba2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pregnenolone + NAD(P)+ Homo sapiens
-
pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
Homo sapiens 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
?
pregnenolone + NAD(P)+
-
Homo sapiens pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
30 min, 50% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ more than 10fold as active as NADP+ Homo sapiens
NADP+ 10times less effective than NAD+ Homo sapiens