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Literature summary for 1.1.1.14 extracted from

  • Klimacek, M.; Hellmer, H.; Nidetzky, B.
    Catalytic mechanism of Zn2+-dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu154-Cys forms of yeast xylitol dehydrogenase (2007), Biochem. J., 404, 421-429.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida sp. HA 167

Protein Variants

Protein Variants Comment Organism
E154C purified preparations of mutant contain 0.1-0.4 atoms of Zn2+ per subunit and exhibit a constant catalytic Zn2+ centre activity of 1.19 per s, mutant does not require exogenous Zn2+ for stability. Mutant retains less than 1% of wild-type catalytic efficiency and displays similar primary and solvent deuterium effects as wild-type Candida sp. HA 167

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 NAD+ mutant E154C, pH 9.0, 25°C Candida sp. HA 167
0.43
-
 NAD+ wild-type, pH 9.0, 25°C Candida sp. HA 167
0.5
-
 NAD+ pH 9.0, 25°C Ovis aries
3
-
 D-sorbitol pH 9.0, 25°C Ovis aries
21
-
 D-sorbitol wild-type, pH 9.0, 25°C Candida sp. HA 167
785
-
 D-sorbitol mutant E154C, pH 9.0, 25°C Candida sp. HA 167

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Ovis aries
Zn2+ mutant E154C conatins 0.1-0.4 atoms per subunit Candida sp. HA 167

Organism

Organism UniProt Comment Textmining
Candida sp. HA 167
-
-
-
Ovis aries
-
 commercial preparation
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+
-
 Ovis aries D-fructose + NADH + H+
-
 ?
D-sorbitol + NAD+
-
 Candida sp. HA 167 D-fructose + NADH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.19
-
 D-sorbitol mutant E154C, pH 9.0, 25°C Candida sp. HA 167
2 8 D-sorbitol pH 9.0, 25°C Ovis aries
161
-
 D-sorbitol wild-type, pH 9.0, 25°C Candida sp. HA 167

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ovis aries
NAD+
-
 Candida sp. HA 167
NADH
-
 Ovis aries
NADH
-
 Candida sp. HA 167