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Literature summary for 1.1.1.14 extracted from

  • Adachi, O.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.
    Crystallization and properties of NAD-dependent D-sorbitol dehydrogenase from Gluconobacter suboxydans IFO 3257 (1999), Biosci. Biotechnol. Biochem., 63, 1589-1595.
    View publication on PubMed
Search for more literature for 1.1.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 NAD+
-
 Gluconobacter oxydans
0.28
-
 NADH
-
 Gluconobacter oxydans
5
-
 D-sorbitol
-
 Gluconobacter oxydans
20
-
 D-fructose
-
 Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 4 * 26000, SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans IFO3257
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136.5
-
-
 Gluconobacter oxydans

Storage Stability

Storage Stability Organism
4°C several days Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Gluconobacter oxydans D-sorbitol + NAD+
-
 ?
D-fructose + NADH + H+
-
 Gluconobacter oxydans IFO3257 D-sorbitol + NAD+
-
 ?
D-mannitol + NAD+ low activity Gluconobacter oxydans ?
-
 ?
D-mannitol + NAD+ low activity Gluconobacter oxydans IFO3257 ?
-
 ?
D-sorbitol + NAD+
-
 Gluconobacter oxydans D-fructose + NADH + H+
-
 r
D-sorbitol + NAD+
-
 Gluconobacter oxydans IFO3257 D-fructose + NADH + H+
-
 r
D-xylitol + NAD+
-
 Gluconobacter oxydans ?
-
 ?
D-xylitol + NAD+
-
 Gluconobacter oxydans IFO3257 ?
-
 ?
D-xylulose + NADH
-
 Gluconobacter oxydans ?
-
 ?
D-xylulose + NADH
-
 Gluconobacter oxydans IFO3257 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 26000, SDS-PAGE Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable Gluconobacter oxydans
50
-
 denaturation after 5 min Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 D-fructose reduction Gluconobacter oxydans
9 10.5 sorbitol oxidation Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 11 several days at 4°C Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Gluconobacter oxydans
NADH
-
 Gluconobacter oxydans