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Literature summary for 1.1.1.14 extracted from

  • Takayuki, U.
    Purification and properties of NAD+-dependent sorbitol dehydrogenase from Bacillus fructosus (1999), Biosci. Biotechnol. Biochem., 63, 573-574.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Priestia megaterium
Cu2+
-
Priestia megaterium
Hg2+
-
Priestia megaterium
p-chloromercuribenzoate
-
Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
NAD+
-
Priestia megaterium
11
-
D-sorbitol
-
Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
-
Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+
-
Priestia megaterium D-fructose + NADH + H+
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, stable Priestia megaterium
50
-
5% loss of activity after 30 min Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 30 min at 30°C Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Priestia megaterium
NADH
-
Priestia megaterium