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Literature summary for 1.1.1.14 extracted from

  • Marini, I.; Bucchioni, L.; Borella, P.; Del Corso, A.; Mura, U.
    Sorbitol dehydrogenase from bovine lens: purification and properties (1997), Arch. Biochem. Biophys., 340, 383-391.
    View publication on PubMed
Search for more literature for 1.1.1.14

General Stability

General Stability Organism
freezing, loss of activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Bos taurus
EDTA
-
 Bos taurus
quercetin
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 NADH
-
 Bos taurus
0.089
-
 NAD+
-
 Bos taurus
3.2
-
 D-xylitol
-
 Bos taurus
18
-
 D-sorbitol
-
 Bos taurus
20
-
 L-Iditol
-
 Bos taurus
67
-
 D-ribitol
-
 Bos taurus
180
-
 D-fructose
-
 Bos taurus
260
-
 D-ribulose
-
 Bos taurus
268
-
 L-Threitol
-
 Bos taurus
381
-
 D-mannitol
-
 Bos taurus
670
-
 L-sorbose
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn 0.83 mol per subunit Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 4 * 39000, SDS-PAGE Bos taurus
156000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, 2 mM dithiothreitol, 0.1 mM NADH, 15 days Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Bos taurus D-sorbitol + NAD+
-
 ?
D-mannitol + NAD+ low activity Bos taurus ?
-
 ?
D-ribitol + NAD+
-
 Bos taurus ?
-
 ?
D-ribulose + NADH
-
 Bos taurus ?
-
 ?
D-sorbitol + NAD+
-
 Bos taurus D-fructose + NADH + H+
-
 ?
D-xylitol + NAD+
-
 Bos taurus ?
-
 ?
L-iditol + NAD+
-
 Bos taurus ?
-
 ?
L-sorbose + NADH
-
 Bos taurus ?
-
 ?
L-threitol + NAD+ low activity Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 D-fructose reduction Bos taurus
9
-
 sorbitol oxidation Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Bos taurus
NADH specific for Bos taurus