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Literature summary for 1.1.1.14 extracted from

  • Schauder, S.; Schneider, K.H.; Giffhorn, F.
    Polyol metabolism of Rhodobacter sphaeroides: biochemical characterization of a short-chain sorbitol dehydrogenase (1995), Microbiology, 141, 1857-1863.
    View publication on PubMed
Search for more literature for 1.1.1.14

General Stability

General Stability Organism
1 mM dithiothreitol reduces stability Cereibacter sphaeroides
30% sucrose stabilizes Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Cereibacter sphaeroides
N-bromosuccinimide
-
 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NADH
-
 Cereibacter sphaeroides
0.13
-
 NADH
-
 Cereibacter sphaeroides
1.5
-
 D-galactitol
-
 Cereibacter sphaeroides
6.2
-
 D-glucitol
-
 Cereibacter sphaeroides
13
-
 D-tagatose
-
 Cereibacter sphaeroides
160
-
 D-fructose
-
 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, SDS-PAGE Cereibacter sphaeroides
61000
-
 Stokes' radius, sedimentation coefficient Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides Si4 / DSM 8371
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.3
-
-
 Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
4°C, 20 mM potassium phosphate buffer, pH 7.0, 3 months Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+ D-arabinitol is identical, low activity Cereibacter sphaeroides ?
-
 ?
D-arabitol + NAD+ D-arabinitol is identical, low activity Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
D-fructose + NADH + H+
-
 Cereibacter sphaeroides D-sorbitol + NAD+
-
 ?
D-galactitol + NAD+
-
 Cereibacter sphaeroides D-tagatose + NADH
-
 r
D-glucitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 r
D-mannitol + NAD+ low activity Cereibacter sphaeroides ?
-
 ?
D-sorbitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 r
D-sorbitol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 D-fructose + NADH + H+
-
 r
D-tagatose + NADH
-
 Cereibacter sphaeroides D-galactitol + NAD+
-
 r
D-xylitol + NAD+ low activity Cereibacter sphaeroides ?
-
 ?
D-xylulose + NADH low activity Cereibacter sphaeroides ?
-
 ?
D-xylulose + NADH low activity Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
L-arabitol + NAD+
-
 Cereibacter sphaeroides ?
-
 ?
L-arabitol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
L-iditol + NAD+
-
 Cereibacter sphaeroides ?
-
 ?
L-iditol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
L-sorbose + NADH low activity Cereibacter sphaeroides ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 50% loss of activity after 8 days in presence of 30% sucrose Cereibacter sphaeroides
40
-
 50% loss of activity after 18 hours in presence of 30% sucrose Cereibacter sphaeroides
50
-
 50% loss of activity after 2 min in presence of 30% sucrose Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.2 D-fructose reduction Cereibacter sphaeroides
11
-
 sorbitol oxidation Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
6 7.2 less than 50% of maximal activity above and below, sugar reduction Cereibacter sphaeroides
9.2 11.6 less than 50% of maximal activity above and below, sorbitol oxidation Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Cereibacter sphaeroides
NADH specific for Cereibacter sphaeroides