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Literature summary for 1.1.1.14 extracted from

  • Schneider, K.H.; Giffhorn, F.
    Sorbitol dehydrogenase from Pseudomonas sp.: Purification, characterization and application to quantitative determination of sorbitol (1991), Enzyme Microb. Technol., 13, 332-337.
No PubMed abstract available
Search for more literature for 1.1.1.14

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM 24% stimulation Pseudomonas sp.

General Stability

General Stability Organism
30% sucrose stabilizes Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
CdCl2 1 mM Pseudomonas sp.
CoCl2 1 mM Pseudomonas sp.
CuSO4 1 mM Pseudomonas sp.
DL-2-bromosuccinate 1 mM Pseudomonas sp.
FeSO4 1 mM Pseudomonas sp.
MgSO4 1 mM Pseudomonas sp.
N-ethylmaleimide 1 mM Pseudomonas sp.
NiCl2 1 mM Pseudomonas sp.
p-chloromercuribenzoate 1 mM Pseudomonas sp.
ZnSO4 1 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NADH
-
 Pseudomonas sp.
0.24
-
 NAD+
-
 Pseudomonas sp.
3.1
-
 D-galactitol
-
 Pseudomonas sp.
9.1
-
 D-glucitol
-
 Pseudomonas sp.
10
-
 D-tagatose
-
 Pseudomonas sp.
176
-
 D-fructose
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32300
-
 2 * 32300, SDS-PAGE Pseudomonas sp.
65800
-
 Stokes' radius, sedimentation coefficient Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
89.5
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NADH low activity Pseudomonas sp. ?
-
 ?
D-arabitol + NAD+ D-arabinitol is identical, low activity Pseudomonas sp. ?
-
 ?
D-fructose + NADH + H+
-
 Pseudomonas sp. D-sorbitol + NAD+
-
 ?
D-galactitol + NAD+
-
 Pseudomonas sp. D-tagatose + NADH
-
 r
D-glucitol + NAD+
-
 Pseudomonas sp. D-fructose + NADH + H+
-
 r
D-mannitol + NAD+ low activity Pseudomonas sp. ?
-
 ?
D-mannose + NADH low activity Pseudomonas sp. ?
-
 ?
D-sorbitol + NAD+
-
 Pseudomonas sp. D-fructose + NADH + H+
-
 r
D-tagatose + NADH
-
 Pseudomonas sp. D-galactitol + NAD+
-
 r
D-xylitol + NAD+ low activity Pseudomonas sp. ?
-
 ?
D-xylulose + NADH low activity Pseudomonas sp. ?
-
 ?
L-iditol + NAD+
-
 Pseudomonas sp. ?
-
 ?
L-sorbose + NADH low activity Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32300, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 50% loss of activity after 4 days Pseudomonas sp.
20
-
 50% loss of activity after 32 hours Pseudomonas sp.
30
-
 50% loss of activity after 6 hours Pseudomonas sp.
40
-
 50% loss of activity after 5 min Pseudomonas sp.
50
-
 50% loss of activity after 1 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 D-fructose reduction Pseudomonas sp.
11
-
 sorbitol oxidation Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.
NADH
-
 Pseudomonas sp.