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Literature summary for 1.1.1.14 extracted from
- Sorbitol dehydrogenase from horse liver: Purification, characterization and comparative properties (1981), Comp. Biochem. Physiol. B, 69, 909-914.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | above 7 mM, substrate inhibition | Equus caballus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
NADH | - |
Equus caballus | |
| 0.041 | - |
NAD+ | - |
Equus caballus | |
| 0.24 | - |
D-sorbitol | - |
Equus caballus | |
| 330 | - |
D-fructose | - |
Equus caballus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Ovis aries |
| 26000 | - |
x * 26000, SDS-PAGE | Equus caballus |
| 106000 | - |
gel filtration | Ovis aries |
| 110000 | - |
gel filtration | Equus caballus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Equus caballus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries | - |
| liver | - |
Equus caballus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 4 months | Equus caballus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | - |
Ovis aries | D-sorbitol + NAD+ | - |
? | |
| D-fructose + NADH + H+ | - |
Equus caballus | D-sorbitol + NAD+ | - |
? | |
| D-galactitol + NAD+ | low activity | Equus caballus | D-tagatose + NADH | - |
? | |
| D-mannitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| D-mannitol + NAD+ | - |
Equus caballus | ? | - |
? | |
| D-ribitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| D-ribitol + NAD+ | - |
Equus caballus | ? | - |
? | |
| D-sorbitol + NAD+ | - |
Ovis aries | D-fructose + NADH + H+ | - |
? | |
| D-sorbitol + NAD+ | - |
Equus caballus | D-fructose + NADH + H+ | - |
? | |
| D-xylitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| D-xylitol + NAD+ | - |
Equus caballus | ? | - |
? | |
| L-arabitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| L-arabitol + NAD+ | low activity | Equus caballus | ? | - |
? | |
| L-iditol + NAD+ | - |
Ovis aries | ? | - |
? | |
| L-iditol + NAD+ | - |
Equus caballus | ? | - |
? | |
| L-sorbose + NADH | - |
Equus caballus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Ovis aries |
| ? | x * 26000, SDS-PAGE | Equus caballus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
30% loss of activity after 3 days, 70% loss of activity after 6 days, 100% loss of activity after 10 days | Equus caballus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
D-fructose reduction | Equus caballus |
| 9.6 | - |
sorbitol oxidation | Equus caballus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Ovis aries | |
| NAD+ | specific for | Equus caballus | |
| NADH | - |
Ovis aries | |
| NADH | specific for | Equus caballus | |
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