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Literature summary for 1.1.1.14 extracted from
- Partial purification and characterization of sorbitol dehydrogenase from rat brain (1977), J. Neurochem., 28, 655-660.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | fructose reduction at pH 7.4, inhibits at pH 9.0 | Rattus norvegicus |  |
| ZnSO4 | 3.5 mM reverses EDTA inhibition | Rattus norvegicus | |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes at 4°C | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dimercaptopropanol | - |
Rattus norvegicus | |
| cysteine | - |
Rattus norvegicus | |
| EDTA | at pH 7.4, not at pH 9.0, fructose reduction | Rattus norvegicus | |
| iodoacetate | - |
Rattus norvegicus | |
| p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
D-sorbitol | - |
Rattus norvegicus | |
| 0.4 | - |
D-xylitol | - |
Rattus norvegicus | |
| 1.3 | - |
D-ribitol | - |
Rattus norvegicus | |
| 16 | - |
D-mannitol | - |
Rattus norvegicus | |
| 16.7 | - |
D-galactitol | - |
Rattus norvegicus | |
| 20 | - |
L-arabitol | - |
Rattus norvegicus | |
| 54 | - |
D-ribulose | - |
Rattus norvegicus | |
| 77 | - |
D-fructose | - |
Rattus norvegicus | |
| 110 | - |
L-sorbose | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sorbitol + NAD+ | Rattus norvegicus | - |
D-fructose + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, good preservation | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | - |
Rattus norvegicus | D-sorbitol + NAD+ | - |
? | |
| D-galactitol + NAD+ | - |
Rattus norvegicus | D-tagatose + NADH | - |
? | |
| D-mannitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| D-ribitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| D-ribulose + NADH | - |
Rattus norvegicus | ? | - |
? | |
| D-sorbitol + NAD+ | - |
Rattus norvegicus | D-fructose + NADH + H+ | - |
? | |
| D-xylitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| L-arabitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| L-sorbose + NADH | - |
Rattus norvegicus | ? | - |
? | |
| sorbitol + NAD+ | - |
Rattus norvegicus | D-fructose + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | D-fructose reduction | Rattus norvegicus |
| 9 | 10 | sorbitol oxidation | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | at pH 6 about 70% of maximal activity, at pH 9 about 40%, fructose reduction | Rattus norvegicus |
| 7.5 | 10 | at pH 7.5 about 70% of maximal activity, at pH 10 about 100%, sorbitol oxidation | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
| NADP+ | cannot replace NAD+ | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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