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Literature summary for 1.1.1.14 extracted from
- Detection and characterization of sorbitol dehydrogenase from apple callus tissue (1979), Plant Physiol., 64, 69-73.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ZnSO4 | reverses cysteine inhibition | Malus domestica |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Malus domestica | |
| cysteine | strong effect on sorbitol oxidation, slight effect on fructose reduction, ZnSO4 reverses inhibition | Malus domestica | |
| HgCl2 | - |
Malus domestica | |
| iodoacetate | strong effect on sorbitol oxidation, weaker effect on fructose reduction | Malus domestica | |
| p-chloromercuribenzoate | - |
Malus domestica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 37 | - |
D-xylitol | - |
Malus domestica | |
| 86 | - |
D-sorbitol | - |
Malus domestica | |
| 1500 | - |
D-fructose | - |
Malus domestica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
apple | - |
| Malus domestica | - |
cv Granny Smith | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mesocarp | tissue culture | Malus domestica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | - |
Malus domestica | D-sorbitol + NAD+ | - |
? | |
| D-mannitol + NAD+ | - |
Malus domestica | ? | - |
? | |
| D-ribitol + NAD+ | - |
Malus domestica | ? | - |
? | |
| D-sorbitol + NAD+ | - |
Malus domestica | D-fructose + NADH + H+ | - |
? | |
| D-xylitol + NAD+ | - |
Malus domestica | ? | - |
? | |
| L-arabitol + NAD+ | - |
Malus domestica | ? | - |
? | |
| L-sorbose + NADH | - |
Malus domestica | ? | - |
? | |
| L-threitol + NAD+ | - |
Malus domestica | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Malus domestica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | 35°C: about 50% of activity maximum, 65°C: about 95% of activity maximum | Malus domestica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 7 | D-fructose reduction | Malus domestica |
| 9 | 10 | sorbitol oxidation | Malus domestica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | 8 | at pH 4.7 and 8 about 50% of activity maximum, D-fructose reduction | Malus domestica |
| 8 | 11 | at pH 8 and 11 about 50% of activity maximum, sorbitol oxidation | Malus domestica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
at lower pH denaturation occurs | Malus domestica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Malus domestica | |
| NADH | - |
Malus domestica | |
| NADP+ | cannot replace NAD+ | Malus domestica | |
| NADPH | less effective than NADH | Malus domestica | |
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