Any feedback?
Literature summary for 1.1.1.14 extracted from
- Sorbitol dehydrogenase from rat liver (1982), Methods Enzymol., 89, 135-140.
General Stability
| General Stability | Organism |
|---|---|
| 0.1 mg/ml bovine serum albumin stabilizes at 4°C | Rattus norvegicus |
| 278 mM maltose stabilizes at 4°C | Rattus norvegicus |
| 6.8 M glycerol stabilizes at 4°C | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | above 250 mM, substrate inhibition | Rattus norvegicus |  |
| D-sorbitol | above 49 mM, substrate inhibition | Rattus norvegicus | |
| NAD+ | above 7 mM, substrate inhibition | Rattus norvegicus | |
| NADH | above 0.4 mM, substrate inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
NADH | - |
Rattus norvegicus | |
| 0.082 | - |
NAD+ | - |
Rattus norvegicus | |
| 0.38 | - |
D-sorbitol | - |
Rattus norvegicus | |
| 136 | - |
D-fructose | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Rattus norvegicus |
| 97000 | - |
disc gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.47 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabitol + NAD+ | D-arabinitol is identical, low activity | Rattus norvegicus | ? | - |
? | |
| D-arabitol + NAD+ | D-arabinitol is identical, low activity | Ovis aries | ? | - |
? | |
| D-fructose + NADH + H+ | - |
Rattus norvegicus | D-sorbitol + NAD+ | - |
? | |
| D-fructose + NADH + H+ | - |
Ovis aries | D-sorbitol + NAD+ | - |
? | |
| D-mannitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| D-mannitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| D-ribitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| D-ribitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| D-sorbitol + NAD+ | - |
Rattus norvegicus | D-fructose + NADH + H+ | - |
? | |
| D-sorbitol + NAD+ | - |
Ovis aries | D-fructose + NADH + H+ | - |
? | |
| D-xylitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
| D-xylitol + NAD+ | - |
Ovis aries | ? | - |
? | |
| L-sorbose + NADH | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000-40000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
5 h, stable | Rattus norvegicus |
| 60 | - |
10 min, 65% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
D-fructose reduction | Rattus norvegicus |
| 8.1 | 8.5 | sorbitol oxidation | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Ovis aries | |
| NAD+ | specific for | Rattus norvegicus | |
| NADH | - |
Ovis aries | |
| NADH | specific for | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
