Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | D-fructose reduction, NADP+/NADPH ratio of 38 necessary for 50% inhibition | Agaricus bisporus | |
NADPH | D-mannitol oxidation, NADPH/NADP+ ratio of 0.1 necessary for 50% inhibition | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NADP+ | pH 7.0 | Agaricus bisporus | |
0.0385 | - |
NADPH | pH 7.0 | Agaricus bisporus | |
0.082 | - |
NADP+ | pH 10.25 | Agaricus bisporus | |
16.2 | - |
D-mannitol | pH 10.25 and pH 7.0 | Agaricus bisporus | |
1200 | - |
D-fructose | at pH 7.0 | Agaricus bisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
4 * 29500, SDS-PAGE | Agaricus bisporus |
115000 | 130000 | gel filtration, rate zonal ultracentrifugation | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | Agaricus bisporus | - |
D-mannitol + NADP+ | - |
r | |
D-mannitol + NADP+ | Agaricus bisporus | - |
D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Agaricus bisporus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
194.4 | - |
- |
Agaricus bisporus |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.05 mM PMSF, 50%, v/v, glycerol, 1 month | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | - |
Agaricus bisporus | D-mannitol + NADP+ | - |
r | |
D-mannitol + NADP+ | - |
Agaricus bisporus | D-fructose + NADPH + H+ | - |
r | |
L-sorbitol + NADP+ | 7.7% of the activity compared to D-mannitol | Agaricus bisporus | L-sorbose + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29500, SDS-PAGE | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
D-fructose reduction | Agaricus bisporus |
10.25 | - |
D-mannitol oxidation | Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cannot be replaced by NAD+ | Agaricus bisporus | |
NADPH | cannot be replaced by NADH | Agaricus bisporus |