Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | and the particulate fraction required for full activity | Micrococcus luteus |
General Stability | Organism |
---|---|
ammonium sulfate protects against inactivation during storage and incubation | Micrococcus luteus |
monovalent cations protect against inactivation during incubation | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | 10 mM, more than 90% inhibition | Micrococcus luteus | |
N-acetylglucosamine 1-phosphate | - |
Micrococcus luteus | |
p-chloromercuribenzoate | 0.027 mM, complete inhibition | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
UDP-N-acetyl-D-glucosamine | - |
Micrococcus luteus | |
1.43 | - |
NAD+ | - |
Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O | Micrococcus luteus | - |
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
ATCC 4698 | - |
Purification (Comment) | Organism |
---|---|
partial | Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41 | - |
- |
Micrococcus luteus |
Storage Stability | Organism |
---|---|
4°C, 1 M (NH4)2SO4, stable for more than 3 weeks | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O | - |
Micrococcus luteus | UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
above, Tris buffer | Micrococcus luteus |
10 | - |
above, glycine-NaOH buffer | Micrococcus luteus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Micrococcus luteus |