Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrazine | inhibitory at 100 mM | Pseudomonas sp. | |
hydroxylamine | inhibitory at 100 mM | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonate + NAD+ | Pseudomonas sp. | - |
3-dehydro-L-threonate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
100fold purification, calcium phosphate gel | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by growing in L-threonate-containing medium | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
124 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-15°C, 30 mM Tris-HCl, pH 7.5, 5 mM 2-mercaptoethanol, 0.5 M NaCl, 44% retaining activity after 8 weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonate + NAD+ | - |
Pseudomonas sp. | 3-dehydro-L-threonate + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.8 | - |
oxidation reaction | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.9 | 11.5 | 50% of maximal activity at pH 8.9 and pH 11.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cannot be replaced by NADP+ | Pseudomonas sp. |