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Literature summary for 1.1.1.129 extracted from

  • Aspen, A.J.; Jakoby, W.B.
    L-threonic acid dehydrogenase: Purification and properties (1964), J. Biol. Chem., 239, 710-713.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydrazine inhibitory at 100 mM Pseudomonas sp.
hydroxylamine inhibitory at 100 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonate + NAD+ Pseudomonas sp.
-
3-dehydro-L-threonate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
100fold purification, calcium phosphate gel Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by growing in L-threonate-containing medium Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
124
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-15°C, 30 mM Tris-HCl, pH 7.5, 5 mM 2-mercaptoethanol, 0.5 M NaCl, 44% retaining activity after 8 weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonate + NAD+
-
Pseudomonas sp. 3-dehydro-L-threonate + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.8
-
oxidation reaction Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.9 11.5 50% of maximal activity at pH 8.9 and pH 11.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ cannot be replaced by NADP+ Pseudomonas sp.