General Stability | Organism |
---|---|
1 mM NAD+ or NADP+ increase stability | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | Pseudomonas sp. | irreversible at pH 7.0 | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.5 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
0-3°C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | irreversible at pH 7.0 | Pseudomonas sp. | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. | |
NADP+ | - |
Pseudomonas sp. |