BRENDA - Enzyme Database
show all sequences of 1.1.1.126

The first crystal structure of NAD-dependent 3-dehydro-2-deoxy-D-gluconate dehydrogenase from Thermus thermophilus HB8

Pampa, K.J.; Lokanath, N.K.; Kunishima, N.; Rai, R.V.; Acta Crystallogr. Sect. D 70, 994-1004 (2014)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M bicine pH 9.0, 2% (w/v) dioxane, 10% (w/v) PEG 20 000
Thermus thermophilus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-3-deoxy-D-gluconate + NAD+
Thermus thermophilus
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT20-I column chromatography, and Superdex 75 gel filtration
Thermus thermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-dehydro-3-deoxy-D-gluconate + NAD+
-
739788
Thermus thermophilus
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
-
739788
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
X-ay crystallography
Thermus thermophilus
Synonyms
Synonyms
Commentary
Organism
DDGDH
-
Thermus thermophilus
NAD-dependent 3-dehydro-2-deoxy-D-gluconate dehydrogenase
-
Thermus thermophilus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
this enzyme prefers NAD+ as the physiological cofactor
Thermus thermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
this enzyme prefers NAD+ as the physiological cofactor
Thermus thermophilus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M bicine pH 9.0, 2% (w/v) dioxane, 10% (w/v) PEG 20 000
Thermus thermophilus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-3-deoxy-D-gluconate + NAD+
Thermus thermophilus
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT20-I column chromatography, and Superdex 75 gel filtration
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-dehydro-3-deoxy-D-gluconate + NAD+
-
739788
Thermus thermophilus
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
-
739788
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
X-ay crystallography
Thermus thermophilus
Other publictions for EC 1.1.1.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739788
Pampa
The first crystal structure of ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Acta Crystallogr. Sect. D
70
994-1004
2014
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285819
Preiss
Alginic acid metabolism in bac ...
Pseudomonas sp.
J. Biol. Chem.
237
317-321
1962
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