5-keto-D-Fructose reductase from Gluconobacter cerinus

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

D-fructose

inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM

Gluconobacter cerinus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.002

-

NADPH

-

Gluconobacter cerinus

1.3

-

NADP+

-

Gluconobacter cerinus

4.2

-

5-Keto-D-fructose 1-phosphate

-

Gluconobacter cerinus

4.5

-

5-keto-D-fructose

-

Gluconobacter cerinus

70

-

D-fructose

-

Gluconobacter cerinus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

D-fructose + NADP+

Gluconobacter cerinus

predominant formation of D-fructose

5-dehydro-D-fructose + NADPH

-

Gluconobacter cerinus

r

D-fructose + NADP+

Gluconobacter cerinus

highly specific for D-fructose

5-dehydro-D-fructose + NADPH

-

Gluconobacter cerinus

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

5-keto-D-fructose 1-phosphate + NADPH + H+

16% of the rate compared to 5-keto-D-fructose

285816

Gluconobacter cerinus

D-fructose 1-phosphate + NADP+

-

285816

Gluconobacter cerinus

?

D-fructose + NADP+

predominant formation of D-fructose

285816

Gluconobacter cerinus

5-dehydro-D-fructose + NADPH

-

285816

Gluconobacter cerinus

r

D-fructose + NADP+

highly specific for D-fructose

285816

Gluconobacter cerinus

5-dehydro-D-fructose + NADPH

-

285816

Gluconobacter cerinus

r

Cofactor

Commentary

Organism

Structure

NADPH

cannot be replaced by NADH

Gluconobacter cerinus

Cofactor

Commentary

Organism

Structure

NADPH

cannot be replaced by NADH

Gluconobacter cerinus

Inhibitors

Commentary

Organism

Structure

D-fructose

inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM

Gluconobacter cerinus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.002

-

NADPH

-

Gluconobacter cerinus

1.3

-

NADP+

-

Gluconobacter cerinus

4.2

-

5-Keto-D-fructose 1-phosphate

-

Gluconobacter cerinus

4.5

-

5-keto-D-fructose

-

Gluconobacter cerinus

70

-

D-fructose

-

Gluconobacter cerinus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

D-fructose + NADP+

Gluconobacter cerinus

predominant formation of D-fructose

5-dehydro-D-fructose + NADPH

-

Gluconobacter cerinus

r

D-fructose + NADP+

Gluconobacter cerinus

highly specific for D-fructose

5-dehydro-D-fructose + NADPH

-

Gluconobacter cerinus

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

5-keto-D-fructose 1-phosphate + NADPH + H+

16% of the rate compared to 5-keto-D-fructose

285816

Gluconobacter cerinus

D-fructose 1-phosphate + NADP+

-

285816

Gluconobacter cerinus

?

D-fructose + NADP+

predominant formation of D-fructose

285816

Gluconobacter cerinus

5-dehydro-D-fructose + NADPH

-

285816

Gluconobacter cerinus

r

D-fructose + NADP+

highly specific for D-fructose

285816

Gluconobacter cerinus

5-dehydro-D-fructose + NADPH

-

285816

Gluconobacter cerinus

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

285814

Schrimsher

Purification and characterizat ...

Erwinia sp.

Biochem. J.

253

511-516

1988

-

-

-

-

-

-

3

7

-

-

2

1

-

1

-

-

1

-

-

1

1

1

2

1

-

-

-

-

-

2

-

-

2

-

-

-

-

-

-

2

-

-

-

-

3

-

7

-

-

2

1

-

-

-

1

-

1

1

1

2

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

285815

Ameyama

-

5-keto-D-Fructose reductase of ...

Gluconobacter oxydans

Agric. Biol. Chem.

45

863-869

1981

-

-

-

1

-

-

1

2

-

-

2

1

-

1

-

-

1

-

-

1

2

-

1

1

-

-

-

-

-

2

-

1

1

-

-

-

-

-

-

1

1

-

-

-

1

-

2

-

-

2

1

-

-

-

1

-

1

2

-

1

1

-

-

-

-

2

-

1

-

-

-

-

-

-

-

285816

Englard

-

5-keto-D-Fructose reductase fr ...

Gluconobacter cerinus

Methods Enzymol.

41B

127-131

1978

-

-

-

-

-

-

1

5

-

-

-

2

-

1

-

-

1

-

-

1

1

1

3

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

5

-

-

-

2

-

-

-

1

-

1

1

1

3

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

285817

Avigad

5-Keto-D-fructose. IV. A speci ...

Gluconobacter cerinus

J. Biol. Chem.

241

373-378

1966

-

-

-

-

-

-

1

5

-

-

-

2

-

1

-

-

1

-

-

1

1

1

3

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

5

-

-

-

2

-

-

-

1

-

1

1

1

3

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-