Data extracted from this reference:
Inhibitors
D-fructose
inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM
Gluconobacter cerinus
KM Value [mM]
0.002
NADPH
Gluconobacter cerinus
1.3
NADP+
Gluconobacter cerinus
4.2
5-Keto-D-fructose 1-phosphate
Gluconobacter cerinus
4.5
5-keto-D-fructose
Gluconobacter cerinus
70
D-fructose
Gluconobacter cerinus
Natural Substrates/ Products (Substrates)
D-fructose + NADP+
Gluconobacter cerinus
predominant formation of D-fructose
5-dehydro-D-fructose + NADPH
Gluconobacter cerinus
r
D-fructose + NADP+
Gluconobacter cerinus
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
Gluconobacter cerinus
r
Organism
Purification (Commentary)
high purity
Gluconobacter cerinus
Source Tissue
cell culture
Gluconobacter cerinus
Specific Activity [micromol/min/mg]
627
Gluconobacter cerinus
Storage Stability
4°C, stable for 3-4 weeks
Gluconobacter cerinus
Substrates and Products (Substrate)
5-keto-D-fructose 1-phosphate + NADPH + H+
16% of the rate compared to 5-keto-D-fructose
285816
Gluconobacter cerinus
D-fructose 1-phosphate + NADP+
285816
Gluconobacter cerinus
?
D-fructose + NADP+
predominant formation of D-fructose
285816
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
285816
Gluconobacter cerinus
r
D-fructose + NADP+
highly specific for D-fructose
285816
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
285816
Gluconobacter cerinus
r
pH Optimum
6.5
NADPH oxidation in phosphate buffer
Gluconobacter cerinus
7.4
NADPH oxidation in Tris-HCl buffer
Gluconobacter cerinus
Cofactor
NADPH
cannot be replaced by NADH
Gluconobacter cerinus
Cofactor (protein specific)
NADPH
cannot be replaced by NADH
Gluconobacter cerinus
Inhibitors (protein specific)
D-fructose
inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM
Gluconobacter cerinus
KM Value [mM] (protein specific)
0.002
NADPH
Gluconobacter cerinus
1.3
NADP+
Gluconobacter cerinus
4.2
5-Keto-D-fructose 1-phosphate
Gluconobacter cerinus
4.5
5-keto-D-fructose
Gluconobacter cerinus
70
D-fructose
Gluconobacter cerinus
Natural Substrates/ Products (Substrates) (protein specific)
D-fructose + NADP+
Gluconobacter cerinus
predominant formation of D-fructose
5-dehydro-D-fructose + NADPH
Gluconobacter cerinus
r
D-fructose + NADP+
Gluconobacter cerinus
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
Gluconobacter cerinus
r
Purification (Commentary) (protein specific)
high purity
Gluconobacter cerinus
Source Tissue (protein specific)
cell culture
Gluconobacter cerinus
Specific Activity [micromol/min/mg] (protein specific)
627
Gluconobacter cerinus
Storage Stability (protein specific)
4°C, stable for 3-4 weeks
Gluconobacter cerinus
Substrates and Products (Substrate) (protein specific)
5-keto-D-fructose 1-phosphate + NADPH + H+
16% of the rate compared to 5-keto-D-fructose
285816
Gluconobacter cerinus
D-fructose 1-phosphate + NADP+
285816
Gluconobacter cerinus
?
D-fructose + NADP+
predominant formation of D-fructose
285816
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
285816
Gluconobacter cerinus
r
D-fructose + NADP+
highly specific for D-fructose
285816
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
285816
Gluconobacter cerinus
r
pH Optimum (protein specific)
6.5
NADPH oxidation in phosphate buffer
Gluconobacter cerinus
7.4
NADPH oxidation in Tris-HCl buffer
Gluconobacter cerinus
Other publictions for EC
285814
Schrimsher
Purification and characterizat ...
Erwinia sp.
Biochem. J.
253
511-516
1988
-
-
-
-
-
-
3
7
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
7
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285815
Ameyama
-
5-keto-D-Fructose reductase of ...
Gluconobacter oxydans
Agric. Biol. Chem.
45
863-869
1981
-
-
-
1
-
-
1
2
-
-
2
1
-
1
-
-
1
-
-
1
2
-
1
1
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
1
2
-
1
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
285816
Englard
-
5-keto-D-Fructose reductase fr ...
Gluconobacter cerinus
Methods Enzymol.
41B
127-131
1978
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285817
Avigad
5-Keto-D-fructose. IV. A speci ...
Gluconobacter cerinus
J. Biol. Chem.
241
373-378
1966
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-