Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | non-competitive inhibition | Erwinia sp. | |
NADP+ | competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis | Erwinia sp. | |
NADPH | competitive inhibition of D-fructose oxidation | Erwinia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
NADP+ | - |
Erwinia sp. | |
0.071 | - |
NADPH | - |
Erwinia sp. | |
0.32 | - |
NADH | - |
Erwinia sp. | |
1.97 | - |
NAD+ | - |
Erwinia sp. | |
9.1 | - |
D-fructose | - |
Erwinia sp. | |
12.7 | - |
5-keto-D-fructose | - |
Erwinia sp. | |
140 | - |
D-arabinose | - |
Erwinia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Erwinia sp. |
50000 | - |
gel filtration, sedimentation equilibrium measurement | Erwinia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADP+ | Erwinia sp. | highly specific for D-fructose | 5-dehydro-D-fructose + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erwinia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Erwinia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Erwinia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2450 | - |
- |
Erwinia sp. |
Storage Stability | Organism |
---|---|
-20°C, 30 mM Tris-HCl, pH 7.8, 3 months | Erwinia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-fructose + NADH + H+ | - |
Erwinia sp. | D-fructose + NAD+ | - |
r | |
D-arabinose + NADP+ | 5fold lower activity than for D-fructose | Erwinia sp. | ? + NADPH | - |
? | |
D-fructose + NAD+ | - |
Erwinia sp. | 5-dehydro-D-fructose + NADH + H+ | - |
r | |
D-fructose + NADP+ | highly specific for D-fructose | Erwinia sp. | 5-dehydro-D-fructose + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Erwinia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of 5-keto-D-fructose | Erwinia sp. |
9.5 | - |
oxidation of D-fructose | Erwinia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | substitutes for NADPH | Erwinia sp. | |
NADPH | oxidized and reduced cofactors bind to the same site of the enzyme | Erwinia sp. |