Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive inhibitor | Saccharomyces cerevisiae | |
p-hydroxymercuribenzoate | 0.4 mM and 1 mM inhibit activity to 21% and 41% respectively | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
NADPH | pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.29 | - |
5-Keto-D-fructose 1-phosphate | - |
Saccharomyces cerevisiae | |
1 | - |
5-keto-D-fructose | pH 7.5, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
var. aterneum | - |
Bacillus subtilis | - |
168-2 | - |
Bacillus subtilis 168-2 | - |
168-2 | - |
Brevibacillus brevis | - |
9999 | - |
Cyberlindnera jadinii | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Gluconobacter cerinus | - |
- |
- |
Gluconobacter oxydans | - |
Acetobacter melanogenus | - |
Klebsiella aerogenes | - |
- |
- |
no activity in Bacillus cereus | - |
- |
- |
no activity in Candida tropicalis | - |
- |
- |
no activity in Escherichia coli K12 | - |
- |
- |
Priestia megaterium | - |
- |
- |
Proteus vulgaris | - |
- |
- |
Saccharomyces cerevisiae | - |
Boehringer, baker's and brewer's yeast | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.4 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-keto-D-fructose + NADPH | - |
Saccharomyces cerevisiae | L-sorbose + NADP+ | - |
ir | |
5-keto-D-fructose 1-phosphate + NADPH | low activity, maximal rate of 1.8% that observed for 5-keto-D-fructose | Saccharomyces cerevisiae | L-sorbose 1-phosphate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.5 | Tris-acetate buffer | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.2 | pH 5.5 and 10.2: about 30% of activity maximum | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | stereospecific transfer of hydrogen from the A side of reduced pyridine to the ketohexose substrate | Saccharomyces cerevisiae |