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Literature summary for 1.1.1.122 extracted from
- Isolation and characterization of L-fucose dehydrogenase from rabbit liver (1979), J. Biochem., 86, 1559-1565.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | inhibitory | Oryctolagus cuniculus |  |
| Hg2+ | - |
Oryctolagus cuniculus | |
| iodoacetic acid | 10% inhibition at 1 mM | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | 48% inhibition at 1 mM | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
NAD+ | - |
Oryctolagus cuniculus | |
| 0.15 | - |
6-deoxy-L-galactose | - |
Oryctolagus cuniculus | |
| 1.4 | - |
D-arabinose | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-deoxy-L-galactose + NAD+ | Oryctolagus cuniculus | irreversible because of spontaneous conversion to L-fuconic acid during incubation | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
ir | |
| 6-deoxy-L-galactose + NAD+ | Oryctolagus cuniculus | i.e. L-fucose | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
ir | |
| D-arabinose + NAD+ | Oryctolagus cuniculus | 84% of the activity compared to L-fucose | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-xylose + NAD+ | Oryctolagus cuniculus | 2.1% of the activity compared to L-fucose | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
rabbit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps, preparative gel electrophoresis, affinity chromatography | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.03 | - |
purified enzyme | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, presence of NAD+, 1 year | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-deoxy-L-galactose + NAD+ | irreversible because of spontaneous conversion to L-fuconic acid during incubation | Oryctolagus cuniculus | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
ir | |
| 6-deoxy-L-galactose + NAD+ | i.e. L-fucose | Oryctolagus cuniculus | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
ir | |
| D-arabinose + NAD+ | 84% of the activity compared to L-fucose | Oryctolagus cuniculus | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-xylose + NAD+ | 2.1% of the activity compared to L-fucose | Oryctolagus cuniculus | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life: 30 min | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 12 | stable between | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NAD-specific | Oryctolagus cuniculus | |
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