Literature summary for 1.1.1.119 extracted from

Baker, P.J.; Britton, K.L.; Fisher, M.; Esclapez, J.; Pire, C.; Bonete, M.J.; Ferrer, J.; Rice, D.W.
Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily (2009), Proc. Natl. Acad. Sci. USA, 106, 779-784.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Haloferax mediterranei
the structures of GlcDH in binary complexes with either NADP+/Zn2+ or NADPH/Zn2+, a ternary complex with NADP+/Zn2+/glucose, and a nonproductive ternary complex with NADP+/Zn2+ /gluconolactone are determined from isomorphous crystals, in the presence of zinc (1 mM) and either 5 mM NADP+, by using 1.4-1.6 M sodium citrate as the precipitant (in 100 mM HEPES, pH 7.0, and 2 M NaCl) or 5 mM NADPH, using 67-72% (w/v) 2-methyl-2,4-pentanediol as precipitant (in 100 mM HEPES, pH 7.5, and 2 M NaCl)	Haloferax mediterranei

Crystallization (Comment)

Organism

Haloferax mediterranei

the structures of GlcDH in binary complexes with either NADP+/Zn2+ or NADPH/Zn2+, a ternary complex with NADP+/Zn2+/glucose, and a nonproductive ternary complex with NADP+/Zn2+ /gluconolactone are determined from isomorphous crystals, in the presence of zinc (1 mM) and either 5 mM NADP+, by using 1.4-1.6 M sodium citrate as the precipitant (in 100 mM HEPES, pH 7.0, and 2 M NaCl) or 5 mM NADPH, using 67-72% (w/v) 2-methyl-2,4-pentanediol as precipitant (in 100 mM HEPES, pH 7.5, and 2 M NaCl)

Haloferax mediterranei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent on	Haloferax mediterranei
Zn2+	zinc is an important component of the catalytic centre	Haloferax mediterranei

Organism

Organism	UniProt	Comment	Textmining
Haloferax mediterranei	Q977U7	-	-
Haloferax mediterranei	Q977U7	extremely halophilic archaeon	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + NADP+	-	Haloferax mediterranei	D-glucono-1,5-lactone + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GlcDH	-	Haloferax mediterranei
glucose dehydrogenase	-	Haloferax mediterranei

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Haloferax mediterranei

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Release 2023.1 (January 2023)