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show all sequences of 1.1.1.117

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition

Hu, X.Q.; Guo, P.C.; Ma, J.D.; Li, W.F.; Acta Crystallogr. Sect. F 69, 1190-1195 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Crystallization (Commentary)
Crystallization
Organism
apo and NADPH-complexed enzyme forms, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, 0.1 M HEPES pH 7.5
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinose + NADP+
Saccharomyces cerevisiae
-
D-arabinono-1,4-lactone + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
P38115
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NADP+
-
739803
Saccharomyces cerevisiae
D-arabinono-1,4-lactone + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
x-ray crystallography
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apo and NADPH-complexed enzyme forms, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, 0.1 M HEPES pH 7.5
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinose + NADP+
Saccharomyces cerevisiae
-
D-arabinono-1,4-lactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NADP+
-
739803
Saccharomyces cerevisiae
D-arabinono-1,4-lactone + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
x-ray crystallography
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739803
Hu
Structures of Saccharomyces ce ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. F
69
1190-1195
2013
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1
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10663
Kim
D-Arabinose dehydrogenase and ...
Candida albicans
Biochim. Biophys. Acta
1297
1-8
1996
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7
6
1
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2
1
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1
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1
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5
1
1
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7
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5
1
1
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1
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389443
Metzger
Partial purification of rat li ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
26
742-747
1967
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2
1
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2
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1
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285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
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1
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2
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5
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1
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4
3
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1
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1
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2
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4
3
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1
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1
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285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
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3
1
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1
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5
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1
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5
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1
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1
1
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2
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3
1
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1
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5
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1
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1
1
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285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
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1
1
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3
2
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5
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1
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4
1
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1
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2
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1
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