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show all sequences of 1.1.1.11

Metabolic engineering of the chloroplast genome reveals that the yeast ArDH gene confers enhanced tolerance to salinity and drought in plants

Khan, M.S.; Kanwal, B.; Nazir, S.; Front. Plant Sci. 6, 725 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
the gene can be expressed in agronomic plants to withstand abiotic stresses
Saccharomyces cerevisiae
biotechnology
the gene can be expressed in agronomic plants to withstand abiotic stresses
Saccharomyces cerevisiae
Cloned(Commentary)
Commentary
Organism
recombinant expression of the yeast enzyme in Nicotiana tabacum using the chloroplast transformation vector pMSK83 harboring aadA and ArDH genes under the control of chloroplast regulatory sequences
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-ribulose + NADH + H+
Saccharomyces cerevisiae
-
D-arabinitol + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribulose + NADH + H+
-
740490
Saccharomyces cerevisiae
D-arabinitol + NAD+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Saccharomyces cerevisiae
Application (protein specific)
Application
Commentary
Organism
agriculture
the gene can be expressed in agronomic plants to withstand abiotic stresses
Saccharomyces cerevisiae
biotechnology
the gene can be expressed in agronomic plants to withstand abiotic stresses
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of the yeast enzyme in Nicotiana tabacum using the chloroplast transformation vector pMSK83 harboring aadA and ArDH genes under the control of chloroplast regulatory sequences
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-ribulose + NADH + H+
Saccharomyces cerevisiae
-
D-arabinitol + NAD+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribulose + NADH + H+
-
740490
Saccharomyces cerevisiae
D-arabinitol + NAD+
-
-
-
?
Expression
Organism
Commentary
Expression
Saccharomyces cerevisiae
ArDH enzyme expression in tobacco chloroplasts confers tolerance to NaCl (up to 400 mM). Transgenic plants compared to wild-type survived for only 4-5 weeks on 400 mM NaCl whereas plants remain green and grow normal on concentrations up to 350 mM NaCl. A-week-old seedlings are also challenged with PEG up to 6% in the liquid medium, considering that membranes and proteins are protected under stress conditions due to accumulation of arabitol in chloroplasts. Seedlings are tolerant to 6% PEG, suggesting that ARDH enzyme maintains integrity of membranes in chloroplasts under drought conditions via metabolic engineering
additional information
Expression (protein specific)
Organism
Commentary
Expression
Saccharomyces cerevisiae
ArDH enzyme expression in tobacco chloroplasts confers tolerance to NaCl (up to 400 mM). Transgenic plants compared to wild-type survived for only 4-5 weeks on 400 mM NaCl whereas plants remain green and grow normal on concentrations up to 350 mM NaCl. A-week-old seedlings are also challenged with PEG up to 6% in the liquid medium, considering that membranes and proteins are protected under stress conditions due to accumulation of arabitol in chloroplasts. Seedlings are tolerant to 6% PEG, suggesting that ARDH enzyme maintains integrity of membranes in chloroplasts under drought conditions via metabolic engineering
additional information
Other publictions for EC 1.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740490
Khan
Metabolic engineering of the c ...
Saccharomyces cerevisiae
Front. Plant Sci.
6
725
2015
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701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
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8
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3
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3
1
1
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3
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2
1
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1
6
6
670549
LaFayette
Arabitol dehydrogenase as a se ...
Escherichia coli, Escherichia coli C
Plant Cell Rep.
24
596-602
2005
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1
1
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12
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285753
Murray
Isolation, characterization an ...
Candida tropicalis
Gene
155
123-128
1995
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1
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1
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285754
Hallborn
A short-chain dehydrogenase ge ...
Scheffersomyces stipitis
Yeast
11
839-847
1995
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1
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1
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9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans, Candida albicans B331
J. Bacteriol.
175
6314-6320
1993
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9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
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285747
Neuberger
Purification and properties of ...
Klebsiella aerogenes
Biochem. J.
183
31-42
1979
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285748
Wilson
Regulation of D-xylose and D-a ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
J. Bacteriol.
113
1404-1411
1973
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7
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285749
Fossitt
-
D-Arabitol dehydrogenase ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
Methods Enzymol.
9
184-187
1966
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285750
Wood
Ribitol and D-arabitol utiliza ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
J. Biol. Chem.
236
2190-2195
1961
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285751
Lin
An inducible D-arabitol dehydr ...
Klebsiella aerogenes
J. Biol. Chem.
236
31-36
1961
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