Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Rhizobium sp. |
expressed in Escherichia coli JM109 cells | Xanthomonas translucens |
Protein Variants | Comment | Organism |
---|---|---|
A185G | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
F143A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143C | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143D | inactive | Xanthomonas translucens |
F143G | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143H | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143K | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143N | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143S | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
F143W | inactive | Xanthomonas translucens |
F143Y | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
G188A | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
G233S/A224F | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
I190V/A191G | the mutant shows reduced activity compared to the wild type enzyme | Xanthomonas translucens |
S220G/F221A | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
V187I/G188A | inactive | Rhizobium sp. |
Y140A | inactive | Rhizobium sp. |
Y140C | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
Y140D | inactive | Rhizobium sp. |
Y140F | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
Y140G | inactive | Rhizobium sp. |
Y140H | inactive | Rhizobium sp. |
Y140K | inactive | Rhizobium sp. |
Y140N | inactive | Rhizobium sp. |
Y140S | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
Y140W | the mutant shows reduced activity compared to the wild type enzyme | Rhizobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NAD+ | wild type enzyme, at pH 9.5 and 30°C | Rhizobium sp. | |
0.11 | - |
NAD+ | mutant enzyme S220G/F221A, at pH 9.5 and 30°C | Rhizobium sp. | |
0.13 | - |
NAD+ | mutant enzyme Y140F, at pH 9.5 and 30°C | Rhizobium sp. | |
0.15 | - |
NAD+ | mutant enzyme A185G, at pH 9.5 and 30°C | Rhizobium sp. | |
0.21 | - |
NAD+ | mutant enzyme Y140S, at pH 9.5 and 30°C | Rhizobium sp. | |
0.24 | - |
NAD+ | mutant enzyme G223S/A224F, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.24 | - |
NAD+ | mutant enzyme I190V/A191G, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.24 | - |
NAD+ | mutant enzyme Y140W, at pH 9.5 and 30°C | Rhizobium sp. | |
0.25 | - |
NAD+ | mutant enzyme Y140C, at pH 9.5 and 30°C | Rhizobium sp. | |
0.32 | - |
NAD+ | mutant enzyme F143Y, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.32 | - |
NAD+ | wild type enzyme, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.33 | - |
NAD+ | mutant enzyme F143C, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.33 | - |
NAD+ | mutant enzyme F143H, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.34 | - |
NAD+ | mutant enzyme F143N, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.36 | - |
NAD+ | mutant enzyme F143A, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.37 | - |
NAD+ | mutant enzyme F143G, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.42 | - |
NAD+ | mutant enzyme F143S, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.44 | - |
NAD+ | mutant enzyme G188A, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.88 | - |
NAD+ | mutant enzyme F143K, at pH 9.5 and 30°C | Xanthomonas translucens | |
1.07 | - |
carnitine | wild type enzyme, at pH 9.5 and 30°C | Rhizobium sp. | |
1.21 | - |
carnitine | mutant enzyme S220G/F221A, at pH 9.5 and 30°C | Rhizobium sp. | |
1.61 | - |
carnitine | mutant enzyme Y140F, at pH 9.5 and 30°C | Rhizobium sp. | |
1.72 | - |
carnitine | mutant enzyme A185G, at pH 9.5 and 30°C | Rhizobium sp. | |
10.38 | - |
carnitine | wild type enzyme, at pH 9.5 and 30°C | Xanthomonas translucens | |
18.66 | - |
carnitine | mutant enzyme Y140W, at pH 9.5 and 30°C | Rhizobium sp. | |
96.2 | - |
carnitine | mutant enzyme G223S/A224F, at pH 9.5 and 30°C | Xanthomonas translucens | |
96.2 | - |
carnitine | mutant enzyme I190V/A191G, at pH 9.5 and 30°C | Xanthomonas translucens | |
137 | - |
carnitine | mutant enzyme F143Y, at pH 9.5 and 30°C | Xanthomonas translucens | |
137 | - |
carnitine | mutant enzyme G188A, at pH 9.5 and 30°C | Xanthomonas translucens | |
144.9 | - |
carnitine | mutant enzyme F143C, at pH 9.5 and 30°C | Xanthomonas translucens | |
240.3 | - |
carnitine | mutant enzyme F143H, at pH 9.5 and 30°C | Xanthomonas translucens | |
313.6 | - |
carnitine | mutant enzyme Y140C, at pH 9.5 and 30°C | Rhizobium sp. | |
321.5 | - |
carnitine | mutant enzyme F143K, at pH 9.5 and 30°C | Xanthomonas translucens | |
330.1 | - |
carnitine | mutant enzyme Y140S, at pH 9.5 and 30°C | Rhizobium sp. | |
353.8 | - |
carnitine | mutant enzyme F143A, at pH 9.5 and 30°C | Xanthomonas translucens | |
477.8 | - |
carnitine | mutant enzyme F143N, at pH 9.5 and 30°C | Xanthomonas translucens | |
723.4 | - |
carnitine | mutant enzyme F143S, at pH 9.5 and 30°C | Xanthomonas translucens | |
2955 | - |
carnitine | mutant enzyme F143G, at pH 9.5 and 30°C | Xanthomonas translucens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Xanthomonas translucens |
50000 | - |
x * 50000, SDS-PAGE | Rhizobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carnitine + NAD+ | Rhizobium sp. | - |
3-dehydrocarnitine + NADH + H+ | - |
r | |
carnitine + NAD+ | Xanthomonas translucens | - |
3-dehydrocarnitine + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium sp. | - |
- |
- |
Xanthomonas translucens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Rhizobium sp. |
amylose resin column chromatography | Xanthomonas translucens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carnitine + NAD+ | - |
Rhizobium sp. | 3-dehydrocarnitine + NADH + H+ | - |
r | |
carnitine + NAD+ | - |
Xanthomonas translucens | 3-dehydrocarnitine + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Rhizobium sp. |
? | x * 37000, SDS-PAGE | Xanthomonas translucens |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Rhizobium sp. |
Cdh | - |
Xanthomonas translucens |
L-carnitine dehydrogenase | - |
Rhizobium sp. |
L-carnitine dehydrogenase | - |
Xanthomonas translucens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme retains about 29% of its initial activity after incubation for 30 min at 40°C | Rhizobium sp. |
45 | - |
the enzyme retains 100% activity after incubation for 30 min at 45°C | Xanthomonas translucens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
carnitine | mutant enzyme F143G, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.003 | - |
carnitine | mutant enzyme Y140S, at pH 9.5 and 30°C | Rhizobium sp. | |
0.006 | - |
carnitine | mutant enzyme F143S, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.007 | - |
carnitine | mutant enzyme F143A, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.01 | - |
carnitine | mutant enzyme Y140C, at pH 9.5 and 30°C | Rhizobium sp. | |
0.016 | - |
carnitine | mutant enzyme F143K, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.017 | - |
carnitine | mutant enzyme F143N, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.039 | - |
carnitine | mutant enzyme F143H, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.05 | - |
carnitine | mutant enzyme F143Y, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.051 | - |
carnitine | mutant enzyme F143C, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.236 | - |
carnitine | mutant enzyme Y140W, at pH 9.5 and 30°C | Rhizobium sp. | |
0.37 | - |
carnitine | mutant enzyme G233S/A224F, at pH 9.5 and 30°C | Xanthomonas translucens | |
0.37 | - |
carnitine | mutant enzyme I190V/A191G, at pH 9.5 and 30°C | Xanthomonas translucens | |
1.19 | - |
carnitine | mutant enzyme G188A, at pH 9.5 and 30°C | Xanthomonas translucens | |
1.97 | - |
carnitine | wild type enzyme, at pH 9.5 and 30°C | Xanthomonas translucens | |
6.03 | - |
carnitine | mutant enzyme A185G, at pH 9.5 and 30°C | Rhizobium sp. | |
9.796 | - |
carnitine | mutant enzyme Y140F, at pH 9.5 and 30°C | Rhizobium sp. | |
11.36 | - |
carnitine | mutant enzyme S220G/F221A, at pH 9.5 and 30°C | Rhizobium sp. | |
11.77 | - |
carnitine | wild type enzyme, at pH 9.5 and 30°C | Rhizobium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
the enzyme retains about 14% of its initial activity after incubation for 30 min at pH 4.5 | Rhizobium sp. |
4.5 | - |
the enzyme retains about 14% of its initial activity after incubation for 30 min at pH 4.5 | Xanthomonas translucens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhizobium sp. | |
NAD+ | - |
Xanthomonas translucens |