Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.108 extracted from

  • Eltayeb, M.M.; Mohamed Ahmed, I.A.; Arima, J.; Mori, N.
    Identification of residues essential for the activity and substrate affinity of L-carnitine dehydrogenase (2013), Mol. Biotechnol., 55, 268-276.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Rhizobium sp.
expressed in Escherichia coli JM109 cells Xanthomonas translucens

Protein Variants

Protein Variants Comment Organism
A185G the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.
F143A the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143C the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143D inactive Xanthomonas translucens
F143G the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143H the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143K the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143N the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143S the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
F143W inactive Xanthomonas translucens
F143Y the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
G188A the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
G233S/A224F the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
I190V/A191G the mutant shows reduced activity compared to the wild type enzyme Xanthomonas translucens
S220G/F221A the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.
V187I/G188A inactive Rhizobium sp.
Y140A inactive Rhizobium sp.
Y140C the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.
Y140D inactive Rhizobium sp.
Y140F the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.
Y140G inactive Rhizobium sp.
Y140H inactive Rhizobium sp.
Y140K inactive Rhizobium sp.
Y140N inactive Rhizobium sp.
Y140S the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.
Y140W the mutant shows reduced activity compared to the wild type enzyme Rhizobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
NAD+ wild type enzyme, at pH 9.5 and 30°C Rhizobium sp.
0.11
-
NAD+ mutant enzyme S220G/F221A, at pH 9.5 and 30°C Rhizobium sp.
0.13
-
NAD+ mutant enzyme Y140F, at pH 9.5 and 30°C Rhizobium sp.
0.15
-
NAD+ mutant enzyme A185G, at pH 9.5 and 30°C Rhizobium sp.
0.21
-
NAD+ mutant enzyme Y140S, at pH 9.5 and 30°C Rhizobium sp.
0.24
-
NAD+ mutant enzyme G223S/A224F, at pH 9.5 and 30°C Xanthomonas translucens
0.24
-
NAD+ mutant enzyme I190V/A191G, at pH 9.5 and 30°C Xanthomonas translucens
0.24
-
NAD+ mutant enzyme Y140W, at pH 9.5 and 30°C Rhizobium sp.
0.25
-
NAD+ mutant enzyme Y140C, at pH 9.5 and 30°C Rhizobium sp.
0.32
-
NAD+ mutant enzyme F143Y, at pH 9.5 and 30°C Xanthomonas translucens
0.32
-
NAD+ wild type enzyme, at pH 9.5 and 30°C Xanthomonas translucens
0.33
-
NAD+ mutant enzyme F143C, at pH 9.5 and 30°C Xanthomonas translucens
0.33
-
NAD+ mutant enzyme F143H, at pH 9.5 and 30°C Xanthomonas translucens
0.34
-
NAD+ mutant enzyme F143N, at pH 9.5 and 30°C Xanthomonas translucens
0.36
-
NAD+ mutant enzyme F143A, at pH 9.5 and 30°C Xanthomonas translucens
0.37
-
NAD+ mutant enzyme F143G, at pH 9.5 and 30°C Xanthomonas translucens
0.42
-
NAD+ mutant enzyme F143S, at pH 9.5 and 30°C Xanthomonas translucens
0.44
-
NAD+ mutant enzyme G188A, at pH 9.5 and 30°C Xanthomonas translucens
0.88
-
NAD+ mutant enzyme F143K, at pH 9.5 and 30°C Xanthomonas translucens
1.07
-
carnitine wild type enzyme, at pH 9.5 and 30°C Rhizobium sp.
1.21
-
carnitine mutant enzyme S220G/F221A, at pH 9.5 and 30°C Rhizobium sp.
1.61
-
carnitine mutant enzyme Y140F, at pH 9.5 and 30°C Rhizobium sp.
1.72
-
carnitine mutant enzyme A185G, at pH 9.5 and 30°C Rhizobium sp.
10.38
-
carnitine wild type enzyme, at pH 9.5 and 30°C Xanthomonas translucens
18.66
-
carnitine mutant enzyme Y140W, at pH 9.5 and 30°C Rhizobium sp.
96.2
-
carnitine mutant enzyme G223S/A224F, at pH 9.5 and 30°C Xanthomonas translucens
96.2
-
carnitine mutant enzyme I190V/A191G, at pH 9.5 and 30°C Xanthomonas translucens
137
-
carnitine mutant enzyme F143Y, at pH 9.5 and 30°C Xanthomonas translucens
137
-
carnitine mutant enzyme G188A, at pH 9.5 and 30°C Xanthomonas translucens
144.9
-
carnitine mutant enzyme F143C, at pH 9.5 and 30°C Xanthomonas translucens
240.3
-
carnitine mutant enzyme F143H, at pH 9.5 and 30°C Xanthomonas translucens
313.6
-
carnitine mutant enzyme Y140C, at pH 9.5 and 30°C Rhizobium sp.
321.5
-
carnitine mutant enzyme F143K, at pH 9.5 and 30°C Xanthomonas translucens
330.1
-
carnitine mutant enzyme Y140S, at pH 9.5 and 30°C Rhizobium sp.
353.8
-
carnitine mutant enzyme F143A, at pH 9.5 and 30°C Xanthomonas translucens
477.8
-
carnitine mutant enzyme F143N, at pH 9.5 and 30°C Xanthomonas translucens
723.4
-
carnitine mutant enzyme F143S, at pH 9.5 and 30°C Xanthomonas translucens
2955
-
carnitine mutant enzyme F143G, at pH 9.5 and 30°C Xanthomonas translucens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Xanthomonas translucens
50000
-
x * 50000, SDS-PAGE Rhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carnitine + NAD+ Rhizobium sp.
-
3-dehydrocarnitine + NADH + H+
-
r
carnitine + NAD+ Xanthomonas translucens
-
3-dehydrocarnitine + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Rhizobium sp.
-
-
-
Xanthomonas translucens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Rhizobium sp.
amylose resin column chromatography Xanthomonas translucens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carnitine + NAD+
-
Rhizobium sp. 3-dehydrocarnitine + NADH + H+
-
r
carnitine + NAD+
-
Xanthomonas translucens 3-dehydrocarnitine + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Rhizobium sp.
? x * 37000, SDS-PAGE Xanthomonas translucens

Synonyms

Synonyms Comment Organism
Cdh
-
Rhizobium sp.
Cdh
-
Xanthomonas translucens
L-carnitine dehydrogenase
-
Rhizobium sp.
L-carnitine dehydrogenase
-
Xanthomonas translucens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the enzyme retains about 29% of its initial activity after incubation for 30 min at 40°C Rhizobium sp.
45
-
the enzyme retains 100% activity after incubation for 30 min at 45°C Xanthomonas translucens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
carnitine mutant enzyme F143G, at pH 9.5 and 30°C Xanthomonas translucens
0.003
-
carnitine mutant enzyme Y140S, at pH 9.5 and 30°C Rhizobium sp.
0.006
-
carnitine mutant enzyme F143S, at pH 9.5 and 30°C Xanthomonas translucens
0.007
-
carnitine mutant enzyme F143A, at pH 9.5 and 30°C Xanthomonas translucens
0.01
-
carnitine mutant enzyme Y140C, at pH 9.5 and 30°C Rhizobium sp.
0.016
-
carnitine mutant enzyme F143K, at pH 9.5 and 30°C Xanthomonas translucens
0.017
-
carnitine mutant enzyme F143N, at pH 9.5 and 30°C Xanthomonas translucens
0.039
-
carnitine mutant enzyme F143H, at pH 9.5 and 30°C Xanthomonas translucens
0.05
-
carnitine mutant enzyme F143Y, at pH 9.5 and 30°C Xanthomonas translucens
0.051
-
carnitine mutant enzyme F143C, at pH 9.5 and 30°C Xanthomonas translucens
0.236
-
carnitine mutant enzyme Y140W, at pH 9.5 and 30°C Rhizobium sp.
0.37
-
carnitine mutant enzyme G233S/A224F, at pH 9.5 and 30°C Xanthomonas translucens
0.37
-
carnitine mutant enzyme I190V/A191G, at pH 9.5 and 30°C Xanthomonas translucens
1.19
-
carnitine mutant enzyme G188A, at pH 9.5 and 30°C Xanthomonas translucens
1.97
-
carnitine wild type enzyme, at pH 9.5 and 30°C Xanthomonas translucens
6.03
-
carnitine mutant enzyme A185G, at pH 9.5 and 30°C Rhizobium sp.
9.796
-
carnitine mutant enzyme Y140F, at pH 9.5 and 30°C Rhizobium sp.
11.36
-
carnitine mutant enzyme S220G/F221A, at pH 9.5 and 30°C Rhizobium sp.
11.77
-
carnitine wild type enzyme, at pH 9.5 and 30°C Rhizobium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
the enzyme retains about 14% of its initial activity after incubation for 30 min at pH 4.5 Rhizobium sp.
4.5
-
the enzyme retains about 14% of its initial activity after incubation for 30 min at pH 4.5 Xanthomonas translucens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhizobium sp.
NAD+
-
Xanthomonas translucens