Application | Comment | Organism |
---|---|---|
synthesis | under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h | Kangiella koreensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Kangiella koreensis |
expression in Escherichia coli | Kangiella koreensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not influenced by EDTA | Kangiella koreensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is not affected by monovalent or divalent cations | Kangiella koreensis | |
Zn2+ | contains Zn2+ | Kangiella koreensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
- |
Kangiella koreensis |
40000 | - |
- |
Kangiella koreensis |
40000 | - |
2 * 40000, SDS-PAGE | Kangiella koreensis |
80000 | - |
gel filtration | Kangiella koreensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADH + H+ | Kangiella koreensis | - |
all-trans-retinol + NAD+ | - |
r | |
all-trans-retinal + NADH + H+ | Kangiella koreensis SW-125 | - |
all-trans-retinol + NAD+ | - |
r | |
all-trans-retinol + NAD+ | Kangiella koreensis | - |
all-trans-retinal + NADH + H+ | - |
r | |
all-trans-retinol + NAD+ | Kangiella koreensis SW-125 | - |
all-trans-retinal + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kangiella koreensis | - |
- |
- |
Kangiella koreensis | C7R702 | - |
- |
Kangiella koreensis SW-125 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Kangiella koreensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0213 | - |
pH 6.5, 60°C | Kangiella koreensis |
0.0213 | - |
with all-trans-retinal as substrate, at pH 6.5 and 60°C | Kangiella koreensis |
0.024 | - |
with all-trans-retinol as substrate, at pH 6.5 and 60°C | Kangiella koreensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans retinal + NADH + H+ | 26.8% of the activity with butan-1-ol | Kangiella koreensis | all-trans retinol + NAD+ | - |
? | |
all-trans-retinal + NADH + H+ | - |
Kangiella koreensis | all-trans-retinol + NAD+ | - |
r | |
all-trans-retinal + NADH + H+ | 26% activity compared to butyl alcohol | Kangiella koreensis | all-trans-retinol + NAD+ | - |
r | |
all-trans-retinal + NADH + H+ | - |
Kangiella koreensis SW-125 | all-trans-retinol + NAD+ | - |
r | |
all-trans-retinal + NADH + H+ | 26% activity compared to butyl alcohol | Kangiella koreensis SW-125 | all-trans-retinol + NAD+ | - |
r | |
all-trans-retinol + NAD+ | - |
Kangiella koreensis | all-trans-retinal + NADH + H+ | - |
r | |
all-trans-retinol + NAD+ | 30.2% activity compared to butyl alcohol | Kangiella koreensis | all-trans-retinal + NADH + H+ | - |
? | |
all-trans-retinol + NAD+ | - |
Kangiella koreensis SW-125 | all-trans-retinal + NADH + H+ | - |
r | |
all-trans-retinol + NAD+ | 30.2% activity compared to butyl alcohol | Kangiella koreensis SW-125 | all-trans-retinal + NADH + H+ | - |
? | |
butyl alcohol + NAD+ | 100% activity | Kangiella koreensis | butanal + NADH + H+ | - |
? | |
butyl alcohol + NAD+ | 100% activity | Kangiella koreensis SW-125 | butanal + NADH + H+ | - |
? | |
additional information | enzyme is an alcohol dehydrogenase, EC 1.1.1.1, with activity for all-trans-retinol using NAD+ as a cofactor | Kangiella koreensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE, x * 39900, calculated | Kangiella koreensis |
homodimer | 2 * 40000, SDS-PAGE | Kangiella koreensis |
homodimer | 2 * 39900, cslculated from amino acid sequence | Kangiella koreensis |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Kangiella koreensis |
alcohol dehydrogenase | - |
Kangiella koreensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Kangiella koreensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Kangiella koreensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Kangiella koreensis | |
NADH | - |
Kangiella koreensis | |
NADP+ | - |
Kangiella koreensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.7 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |