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Literature summary for 1.1.1.103 extracted from

  • Higashi, N.; Fukada, H.; Ishikawa, K.
    Kinetic study of thermostable L-threonine dehydrogenase from an archaeon Pyrococcus horikoshii (2005), J. Biosci. Bioeng., 99, 175-180.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3) Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 50 mM Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Pyrococcus horikoshii
0.01
-
NAD+ pH 7.5, 65°C Pyrococcus horikoshii
0.013
-
L-threonine pH 7.5, 65°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.54 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
Cu2+ 0.29 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
Mg2+ 0.2 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
Zn2+ required, 1.22 mol Zn2+ per mol of enzyme subunit Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37742
-
2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation Pyrococcus horikoshii
76000
-
recombinant enzyme, gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Pyrococcus horikoshii
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
orf PH0655, gene TDH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C for 30 min, centrifugation, ion exchange chromatography, and gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Pyrococcus horikoshii (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
L-threonine dehydrogenase
-
Pyrococcus horikoshii
TDH
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 106
-
Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
108
-
2-step thermal inactivation Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2
-
NAD+ pH 7.5, 65°C Pyrococcus horikoshii
7.2
-
L-threonine pH 7.5, 65°C Pyrococcus horikoshii
551.7
-
NAD+ pH 7.5, 65°C Pyrococcus horikoshii
551.7
-
L-threonine pH 7.5, 65°C Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Pyrococcus horikoshii