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Literature summary for 1.1.1.103 extracted from

  • Epperly, B.R.; Dekker, E.E.
    L-Threonine dehydrogenase from Escherichia coli (1991), J. Biol. Chem., 266, 6086-6092.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1.26 mM, 41% inhibition after 1 h, 82% inhibition after 2 h, no change in remaining activity after removal of 1,10-phenanthroline Escherichia coli K-12
dipicolinic acid 40 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+ Escherichia coli K-12
EDTA 50 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+ Escherichia coli K-12
iodoacetate iodoacetate reacts with 1 sulfhydryl group per subunit of the enzyme, enzyme retains 15% of its initial activity Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains 1 Zn2+ atom per subunit Escherichia coli K-12
Zn2+ exchange of Zn2+ with Co2+ or Cd2+ does not change the specific activity of the enzyme Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Escherichia coli K-12
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Escherichia coli K-12 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli K-12