Cloned (Comment) | Organism |
---|---|
FVT1 expressed in CHO cells or yeast | Homo sapiens |
TSC10p expressed in CHO cells or yeast | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A175T | mutation is deleterious to the human enzyme | Homo sapiens |
additional information | mutation of any of the three residues that constitute the catalytic triad of FVT1 do not completely inactivate the enzyme, as it is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C. Deletion of residues 4-26 from the FVT1-GFP protein abolishes ER localization | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | reduces expression of transfected FVT1 mRNA and protein by at least 70% | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | C-terminus of FVT1 | Homo sapiens | 5737 | - |
cytoplasm | N- and C-terminus of Tsc10p | Saccharomyces cerevisiae | 5737 | - |
endoplasmic reticulum | N-terminal membrane-spanning domain in FVT1 orients to place it in the endoplasmic reticulum lumen | Homo sapiens | 5783 | - |
endoplasmic reticulum | Tsc10p localizes to the yeast endoplasmic reticulum membrane | Saccharomyces cerevisiae | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Saccharomyces cerevisiae |
150000 | - |
gel filtration1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydrosphinganine + NADPH + H+ | - |
Homo sapiens | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH + H+ | - |
Saccharomyces cerevisiae | sphinganine + NADP+ | - |
? | |
additional information | FVT1 can replace Tsc10p in yeast | Homo sapiens | ? | - |
? | |
additional information | FVT1 is able to support growth of tsc10DELTA mutant cells at 26°C, but not at 37°C | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration | Homo sapiens |
tetramer | gel filtration | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Fvt1 | - |
Homo sapiens |
Tsc10p | - |
Saccharomyces cerevisiae |