additional information |
transformation with 3-oxoacyl-[acyl-carrier-protein] reductase in antisense orientation, driven by either the cauliflower mosaic virus 35S promoter or a seed-specific acyl carrier protein promoter. In plants with altered reductase activity, total seed yield is reduced in all cases. In less severely affected plant lines, seeds have a normal appearance and composition but the yield of seeds is reduced by approximately 50%. In more severely affected lines, reductions in both seed fatty acid content and the number of seeds produced per plant are evident, resulting in a 90% reduction in fatty acid synthesized per plant. Phenotypes are independent of the promoter used. In severely affected lines, a large proportion of seeds show precocious germination, and these have a reduced oleate content and increased levels of polyunsaturated 18-carbon fatty acids, compared with normal seeds of the same line |
Brassica napus |