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Literature summary for 1.1.1.1 extracted from

  • Kamenskikh, K.; Vekshin, N.
    Reactions of NADH oxidation by tetrazolium and ubiquinone catalyzed by yeast alcohol dehydrogenase (2018), Appl. Biochem. Microbiol., 54, 316-319 .
No PubMed abstract available
Search for more literature for 1.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
ethanol presence of ethanol or isopropanol and alkalization of the medium sharply activates the NADH:p-NTF-reductase reaction, activity with ubiquinone is also activated Saccharomyces cerevisiae
Isopropanol presence of ethanol or isopropanol and alkalization of the medium sharply activates the NADH:p-NTF-reductase reaction, activity with ubiquinone is also activated Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics at different pH values, overview Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00330
-
-
Saccharomyces cerevisiae ATCC 204508 P00330
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrotetrazolium violet + NADH + H+ p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF Saccharomyces cerevisiae a formazan + NAD+
-
 ?
4-nitrotetrazolium violet + NADH + H+ p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF Saccharomyces cerevisiae ATCC 204508 a formazan + NAD+
-
 ?
additional information a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules Saccharomyces cerevisiae ?
-
-
additional information a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules Saccharomyces cerevisiae ATCC 204508 ?
-
-
ubiquinone + NADH + H+
-
 Saccharomyces cerevisiae ubiquinol + NAD+
-
 ?
ubiquinone + NADH + H+
-
 Saccharomyces cerevisiae ATCC 204508 ubiquinol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
ADH
-
 Saccharomyces cerevisiae
ADH1
-
 Saccharomyces cerevisiae
NADH:p-NTF-reductase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Saccharomyces cerevisiae