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Literature summary for 1.1.1.1 extracted from

  • Ying, X.; Wang, Y.; Xiong, B.; Wu, T.; Xie, L.; Yu, M.; Wang, Z.
    Characterization of an allylic/benzyl alcohol dehydrogenase from Yokenella sp. strain WZY002, an organism potentially useful for the synthesis of alpha,beta-unsaturated alcohols from allylic aldehydes and ketones (2014), Appl. Environ. Microbiol., 80, 2399-2409.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 123% of initial activity Yokenella sp.
EDTA 1 mM, 110% of initial activity Yokenella sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Yokenella sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, no residual activity Yokenella sp.
Co2+ 1 mM, 19% residual activity Yokenella sp.
Cu2+ 1 mM, no% residual activity Yokenella sp.
Fe2+ 1 mM, 60% residual activity Yokenella sp.
Zn2+ 1 mM, 0.5% residual activity Yokenella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
benzaldehyde pH 6.5, 65°C Yokenella sp.
0.6
-
NADPH pH 6.5, 65°C Yokenella sp.
0.7
-
NADP+ pH 8.0, 55°C Yokenella sp.
3.3
-
(2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
9.1
-
(2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
14
-
benzyl alcohol pH 8.0, 55°C Yokenella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 113% of initial activity Yokenella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36411
-
x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.
37000
-
x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 20% v/v, 24 h, 87% residual activity Yokenella sp.

Organism

Organism UniProt Comment Textmining
Yokenella sp. W6CX26
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Yokenella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-methylpent-2-enal + NADPH + H+ 41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h Yokenella sp. (2E)-2-methylpent-2-en-1-ol + NADP+
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+ 37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h Yokenella sp. (2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
-
?
(2E)-but-2-en-1-ol + NADP+
-
Yokenella sp. (2E)-but-2-enal + NADPH + H+
-
r
(2E)-but-2-enal + NADPH + H+ yield 96% after 12 h Yokenella sp. (2E)-but-2-en-1-ol + NADP+
-
r
(2E)-dec-2-enal + NADPH + H+ 16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h Yokenella sp. (2E)-dec-2-en-1-ol + NADP+
-
?
(2E)-hex-2-enal + NADPH + H+ 41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h Yokenella sp. (2E)-hex-2-en-1-ol + NADP+
-
?
(2E)-oct-2-enal + NADPH + H+ 30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h Yokenella sp. (2E)-oct-2-en-1-ol + NADP+ plus 27% oct-2-enyl ester ?
benzaldehyde + NADPH + H+ 107% of the activity with (2E)-but-2-enal, yield 99% after 12 h Yokenella sp. benzyl alcohol + NADP+
-
r
benzyl alcohol + NADP+
-
Yokenella sp. benzaldehyde + NADPH + H+
-
r
additional information enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes Yokenella sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
oxidation of crotyl aclohol Yokenella sp.
65
-
reduction of crotonaldehyde Yokenella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
half-life 6.2 h Yokenella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
93.3
-
benzyl alcohol pH 8.0, 55°C Yokenella sp.
101
-
(2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
194
-
NADP+ pH 8.0, 55°C Yokenella sp.
360
-
benzaldehyde pH 6.5, 65°C Yokenella sp.
405
-
(2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
433
-
NADPH pH 6.5, 65°C Yokenella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reduction of crotonaldehyde Yokenella sp.
8
-
oxidation of crotyl aclohol Yokenella sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NAD(H) Yokenella sp.
NADPH enzyme strictly requires NADP(H) as a coenzyme Yokenella sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.6
-
benzyl alcohol pH 8.0, 55°C Yokenella sp.
11
-
(2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
122
-
(2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
278
-
NADP+ pH 8.0, 55°C Yokenella sp.
720
-
NADPH pH 6.5, 65°C Yokenella sp.
899
-
benzaldehyde pH 6.5, 65°C Yokenella sp.