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Literature summary for 1.1.1.1 extracted from

  • Marino-Marmolejo, E.N.; De Leon-Rodriguez, A.; de la Rosa, A.P.; Santos, L.
    Heterologous expression and characterization of an alcohol dehydrogenase from the archeon Thermoplasma acidophilum (2009), Mol. Biotechnol., 42, 61-67.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.7
-
ethanol in 100 mM Tris-HCl, at pH 5.0 and 75°C Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ does not have any effect on activity Thermoplasma acidophilum
Zn2+ maximum activity is reached at 0.5 mM Zn2+,Ta1316 ADH is able to tolerate high concentrations of Zn2+ Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, Ta1316 ADH, SDS-PAGE Thermoplasma acidophilum
36010
-
4 * 36010, Ta1316 ADH, calculated from amino acid sequence Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HIM3 strain DSM 1728
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-100 gel filtration Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.6
-
crude extract Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C Thermoplasma acidophilum
628.7
-
purified Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+ 67.7% activity compared to ethanol Thermoplasma acidophilum butanal + NADH + H+
-
?
1-hexanol + NAD+ 15.3% activity compared to ethanol Thermoplasma acidophilum hexanal + NADH + H+
-
?
1-pentanol + NAD+ 19.1% activity compared to ethanol Thermoplasma acidophilum pentanal + NADH + H+
-
?
2-pentanol + NAD+ 5.7% activity compared to ethanol Thermoplasma acidophilum 2-pentanone + NADH + H+
-
?
2-propanol + NAD+ 73.5% activity compared to ethanol Thermoplasma acidophilum 2-propanone + NADH + H+
-
?
ethanol + NAD+ 100% activity Thermoplasma acidophilum acetaldehyde + NADH + H+
-
?
methanol + NAD+ 49.9% activity compared to ethanol Thermoplasma acidophilum formaldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 36000, Ta1316 ADH, SDS-PAGE Thermoplasma acidophilum
homotetramer 4 * 36010, Ta1316 ADH, calculated from amino acid sequence Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
ADH
-
Thermoplasma acidophilum
Ta1316 ADH
-
Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermoplasma acidophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90
-
Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Thermoplasma acidophilum

pH Range

pH Minimum pH Maximum Comment Organism
2 8
-
Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NAD+ shows NAD+ as the preferred co-factor over NADP+ Thermoplasma acidophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoplasma acidophilum Ta1316 ADH, calculated from amino acid sequence
-
5.5