Crystallization (Comment) | Organism |
---|---|
enzyme-NADP+-cofactor complex, X-ray diffraction strcuture determination and analysis, computational structure modeling | Pelophylax perezi |
Protein Variants | Comment | Organism |
---|---|---|
G223D | unaltered cofactor specificity compared to the wild-type enzyme | Pelophylax perezi |
G223D/T224I | highly reduced activity with NADP+ compared to the wild-type enzyme, wild-type-like activity with NAD+ | Pelophylax perezi |
G223D/T224I/H225N | altered cofactor specificity, highly reduced activity with NADP+ compared to the wild-type enzyme, wild-type-like activity with NAD+ | Pelophylax perezi |
H225N | unaltered cofactor specificity compared to the wild-type enzyme | Pelophylax perezi |
T224I | unaltered cofactor specificity compared to the wild-type enzyme | Pelophylax perezi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax perezi | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Pelophylax perezi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and mutant enzymes | Pelophylax perezi |
Synonyms | Comment | Organism |
---|---|---|
ADH8 | - |
Pelophylax perezi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | activity with mutants G223D/T224I and G223D/T224I/H225N | Pelophylax perezi | |
NADH | activity with mutants G223D/T224I and G223D/T224I/H225N | Pelophylax perezi | |
NADP+ | specific for, residues Gly223, Thr224, His225, Leu200, and Lys228 are involved in cofactor binding pocket formation | Pelophylax perezi | |
NADPH | specific for, residues Gly223, Thr224, His225, Leu200, and Lys228 are involved in cofactor binding pocket formation | Pelophylax perezi |