Ligand glycine

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Basic Ligand Information

Molecular Structure
Picture of glycine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C2H5NO2
glycine
DHMQDGOQFOQNFH-UHFFFAOYSA-N
Synonyms:
aminoacetate, aminoacetic acid, DL-glycine, Gly, L-Gly, L-Glycine
Pathway Source
Pathways
MetaCyc
2-amino-3-hydroxycyclopent-2-enone biosynthesis, 2-aminoethylphosphonate degradation III, 4-hydroxy-2-nonenal detoxification, 5-aminoimidazole ribonucleotide biosynthesis I, 5-aminoimidazole ribonucleotide biosynthesis II more


Show all pahtways known for Show all BRENDA pathways known for glycine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (46 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
glycine + phosphate + thioredoxin = acetyl phosphate + ammonia + thioredoxin disulfide
show the reaction diagram
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glycine + H2O + NAD+ = glyoxylate + NH3 + NADH + H+
show the reaction diagram
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glycine + ferricytochrome c = glyoxylate + ferrocytochrome c + NH3
show the reaction diagram
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Gly + H2O + O2 = glyoxylate + NH3 + H2O2
show the reaction diagram
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glycine + pyruvate + NADH + H+ = N-(carboxymethyl)-D-alanine + NAD+ + H2O
show the reaction diagram
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S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + N-methylglycine
show the reaction diagram
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acyl-CoA + glycine = CoA + N-acylglycine
show the reaction diagram
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succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
show the reaction diagram
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choloyl-CoA + glycine = CoA + glycocholate
show the reaction diagram
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
show the reaction diagram
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glycine + glycolaldehyde = L-4-hydroxythreonine
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (43 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(2-amino-1-hydroxyethyl)phosphonate + O2 = glycine + phosphate
show the reaction diagram
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-
acetyl phosphate + ammonia + thioredoxin disulfide = glycine + phosphate + thioredoxin
show the reaction diagram
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-
glyoxylate + NH3 + NADH = glycine + H2O + NAD+
show the reaction diagram
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-
L-ornithine + guanidinoacetate = L-arginine + glycine
show the reaction diagram
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-
glutathione + [Glu(-Cys)]n-Gly = Gly + [Glu(-Cys)]n+1-Gly
show the reaction diagram
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glyoxylate + L-aspartate = glycine + oxaloacetate
show the reaction diagram
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glyoxylate + L-glutamate = glycine + 2-oxoglutarate
show the reaction diagram
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L-alanine + glyoxylate = pyruvate + glycine
show the reaction diagram
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L-serine + glyoxylate = 3-hydroxypyruvate + glycine
show the reaction diagram
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-
an aromatic amino acid + glyoxylate = an aromatic oxo acid + glycine
show the reaction diagram
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L-kynurenine + glyoxylate = 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
show the reaction diagram
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4-aminobutanoate + glyoxylate = succinate semialdehyde + glycine
show the reaction diagram
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-
Gly-tRNAAla + H2O = Gly + tRNAAla
show the reaction diagram
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-
Cys-Gly + H2O = Cys + Gly
show the reaction diagram
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-
Gly-Pro + H2O = Glycine + proline
show the reaction diagram
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Z-Gly-Pro-Leu-Gly-Pro + H2O = Z-Gly-Pro + L-Leu + Gly + L-Pro
show the reaction diagram
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hippurate + H2O = benzoate + glycine
show the reaction diagram
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N-feruloylglycine + H2O = ferulate + glycine
show the reaction diagram
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N-carbamoyl-glycine + H2O = glycine + CO2 + NH3
show the reaction diagram
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guanidinoacetate + H2O = glycine + urea
show the reaction diagram
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D-threonine = glycine + acetaldehyde
show the reaction diagram
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L-Thr = glycine + acetaldehyde
show the reaction diagram
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L-allo-threonine = glycine + acetaldehyde
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (306 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
glycine + O2 = oxoacetic acid + NH3
show the reaction diagram
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glycine + phosphate + thioredoxin = acetyl phosphate + ammonia + thioredoxin disulfide
show the reaction diagram
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glycine + H2O + NAD+ = ? + NH3 + NADH
show the reaction diagram
-
glycine + ferricytochrome c = glyoxylate + ferrocytochrome c + NH3
show the reaction diagram
-
glycine + pyruvate + NADH + H+ = ?
show the reaction diagram
-
Gly + pyruvate + NADH = ?
show the reaction diagram
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Gly + pyruvate + NADH = N-(carboxymethyl)-D-alanine + NAD+
show the reaction diagram
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glycine + 2,6-dichlorophenolindophenol + H2O = ? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
show the reaction diagram
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5-formimidoyltetrahydrofolate + glycine = tetrahydrofolate + N-formimidoglycine
show the reaction diagram
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acetyl-CoA + glycine = CoA + acetylaminoacetate
show the reaction diagram
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Z-Gln-Gly + glycine = ?
show the reaction diagram
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NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
show the reaction diagram
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NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
show the reaction diagram
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glycine + 2-oxoglutarate = glyoxylate + L-glutamate
show the reaction diagram
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2-oxoglutaramate + glycine = L-glutamine + glyoxylate
show the reaction diagram
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pyruvate + glycine = L-alanine + glyoxylate
show the reaction diagram
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glycine + 2-oxoglutarate = glyoxylate + glutamate
show the reaction diagram
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dTDP-4-dehydro-6-deoxy-D-glucose + glycine = dTDP-4-amino-4,6-dideoxy-D-galactose + glyoxylate
show the reaction diagram
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glycine + pyruvate = glyoxylate + L-alanine
show the reaction diagram
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glycine + 4,5-dioxopentanoate = 5-aminolevulinate + glyoxylate
show the reaction diagram
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glycine + 2-oxoglutarate = glyoxalate + L-glutamate
show the reaction diagram
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pyruvate + glycine = L-alanine + glyoxylate
show the reaction diagram
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ATP + glycine = ADP + glycyl phosphate
show the reaction diagram
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ATP + glycine = ?
show the reaction diagram
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glycine + benzaldehyde = L-threo-3-phenylserine + L-erythro-3-phenylserine
show the reaction diagram
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glycine + acetaldehyde = L-threonine
show the reaction diagram
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glycine = ?
show the reaction diagram
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glycine + H2O = ?
show the reaction diagram
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ATP + glycine + tRNAAla = AMP + diphosphate + glycyl-tRNAAla
show the reaction diagram
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ATP + glycine + CoA = ?
show the reaction diagram
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ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + glycine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-glycine
show the reaction diagram
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ATP + L-Glu + Gly = ADP + phosphate + gamma-L-Glu-Gly
show the reaction diagram
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ATP + L-tyrosine + glycine = ? + AMP
show the reaction diagram
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ATP + L-asparagine + glycine = ADP + phosphate + L-asparaginyl-glycine
show the reaction diagram
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2 ATP + 2 L-Val + Gly = 2 ADP + 2 phosphate + L-Val-L-Val-Gly
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (575 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(2-amino-1-hydroxyethyl)phosphonate + O2 = glycine + phosphate
show the reaction diagram
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-
aminoacetaldehyde + NAD+ + H2O = aminoacetate + NADH
show the reaction diagram
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-
aminoacetaldehyde + NAD+ + H2O = aminoacetate + NADH + H+
show the reaction diagram
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-
acetyl phosphate + ammonia + thioredoxin disulfide = glycine + phosphate + thioredoxin
show the reaction diagram
-
glyoxylate + NH3 + NADH = aminoacetate + NAD+ + H2O
show the reaction diagram
-
-
N-acetyl-D-alanine + H2O + O2 = pyruvate + aminoacetate + H2O2
show the reaction diagram
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-
5'-phospho-pyridoxyl-glycine + H2O + O2 = pyridoxal 5'-phosphate + glycine + H2O2
show the reaction diagram
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sarcosine + O2 + H2O = glycine + formaldehyde + H2O2
show the reaction diagram
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-
sarcosine + acceptor + H2O = glycine + formaldehyde + reduced acceptor
show the reaction diagram
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(S)-ureidoglycine + glyoxylate = N-carbamoyl-2-oxoglycine + glycine
show the reaction diagram
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L-alanine + glyoxylate = pyruvate + glycine
show the reaction diagram
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L-ornithine + glyoxylate = DELTA1-pyrroline-5-carboxylate + glycine
show the reaction diagram
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L-asparagine + glyoxylate = 2-oxosuccinamate + glycine
show the reaction diagram
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-
4-aminobutanoate + glyoxylate = 4-oxobutanoate + glycine
show the reaction diagram
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L-3,5,3'-triiodothyronine + oxaloacetate = 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
show the reaction diagram
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L-tryptophan + glyoxylate = 3-indole-2-oxopropanoate + glycine
show the reaction diagram
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L-histidine + glyoxylate = imidazol-5-yl-pyruvate + glycine
show the reaction diagram
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(R)-3-amino-2-methylpropanoate + glyoxylate = 2-methyl-3-oxopropanoate + glycine
show the reaction diagram
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L-tryptophan + oxaloacetate = 3-(1H-indol-3-yl)-2-oxopropanoate + L-glycine
show the reaction diagram
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L-glutamine + glyoxalate = 2-oxoglutaramate + glycine
show the reaction diagram
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L-phenylalanine + glyoxylate = phenylpyruvate + glycine
show the reaction diagram
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taurine + glyoxylate = 2-sulfoacetaldehyde + glycine
show the reaction diagram
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glyoxylate + L-aspartate = glycine + oxaloacetate
show the reaction diagram
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4-aminobutanoate + glyoxylate = succinate semialdehyde + glycine
show the reaction diagram
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L-alanine + glyoxylate = pyruvate + glycine
show the reaction diagram
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ethyl glycine + H2O = ethanol + glycine
show the reaction diagram
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Gly-tRNAAla + H2O = Gly + tRNAAla
show the reaction diagram
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Gly-4-nitroanilide + H2O = Gly + 4-nitroaniline
show the reaction diagram
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Gly-7-amido-4-methylcoumarin + H2O = Gly + 7-amino-4-methylcoumarin
show the reaction diagram
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glycyl 4-nitroanilide + H2O = glycine + 4-nitroaniline
show the reaction diagram
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glycyl 4-nitroanilide + H2O = glycine + 4-nitroaniline
show the reaction diagram
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L-Asp-Gly + H2O = L-Asp + Gly
show the reaction diagram
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D-Ala-Gly + H2O = D-Ala + Gly
show the reaction diagram
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Ala-Pro-Gly + H2O = Ala-Pro + Gly
show the reaction diagram
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Gly-L-Tyr + H2O = Gly + L-Tyr
show the reaction diagram
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glycyl-L-glutamic acid + H2O = glycine + glutamate
show the reaction diagram
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Benzyloxycarbonyl-Pro-Gly + H2O = Benzyloxycarbonyl-Pro + Gly
show the reaction diagram
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Carbobenzoxy-Gly-Pro-Leu-Gly + H2O = Carbobenzoxy-Gly-Pro-Leu + Gly
show the reaction diagram
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Pro-Phe-Lys-Gly + H2O = Pro-Phe-Lys + Gly
show the reaction diagram
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benzyloxycarbonyl-Gly-Gly + H2O = benzyloxycarbonyl-Gly + Gly
show the reaction diagram
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Nalpha, Nepsilon-diacetyl L-Lys-D-Ala-Gly + H2O = Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
show the reaction diagram
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2-aminobenzoyl-Phe-Arg-Gly + H2O = 2-aminobenzoyl-Phe-Arg + Gly
show the reaction diagram
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pyroglutamyl-Gly + H2O = pyroglutamate + Gly
show the reaction diagram
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beta-Asp-Gly + H2O = Asp + Gly
show the reaction diagram
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Gly-beta-naphthylamide + H2O = Gly + beta-naphthylamine
show the reaction diagram
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Gly-4-nitroanilide + H2O = glycine + 4-nitroaniline
show the reaction diagram
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Gly ethyl ester + H2O = Gly + ethanol
show the reaction diagram
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Z-Gly-Pro-Leu-Gly-Pro + H2O = Z-Gly-Pro + L-Leu + Gly + L-Pro
show the reaction diagram
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Gly-Trp-Met-Asp-Phe-NH2 + H2O = Gly + Trp-Met-Asp-Phe-NH2
show the reaction diagram
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Gly-p-nitroanilide + H2O = Gly + p-nitroaniline
show the reaction diagram
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Nalpha-chloroacetylglycine + H2O = glycine + chloroacetate
show the reaction diagram
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N6-glycyl-L-lysine + H2O = L-lysine + glycine
show the reaction diagram
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N-lauroylglycine + H2O = lauroate + glycine
show the reaction diagram
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glycine-beta-naphthylamide + H2O = glycine + 2-naphthylamine
show the reaction diagram
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N-Carbamoyl-Gly + H2O = Gly + NH3 + CO2
show the reaction diagram
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N-Acetyl-Gly + H2O = acetate + Gly
show the reaction diagram
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Glycyl-D-Glu + H2O = Gly + D-Glu
show the reaction diagram
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Gly-D-Asp + H2O = Glycine + Asp
show the reaction diagram
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N-carbamoyl-glycine + H2O = glycine + CO2 + NH3
show the reaction diagram
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glutaryl-glycine + H2O = glutarate + glycine
show the reaction diagram
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guanidinoacetate + H2O = glycine + urea
show the reaction diagram
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aminoacetonitrile + H2O = aminoacetic acid + NH3
show the reaction diagram
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N-phosphoglycine + H2O = glycine + phosphate
show the reaction diagram
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Aminomalonate = Gly + CO2
show the reaction diagram
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alpha-Aminomalonate + pyruvate = Gly + ? + CO2
show the reaction diagram
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allothreonine + ? = Gly + ?
show the reaction diagram
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5-L-glutamyl-glycine = 5-oxoproline + glycine
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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ATP + H2O + glycine/out = ADP + phosphate + glycine/in
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (34 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
slight activation
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activates
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1 mM concentration, 10% activation free protein, 18% activation immobilized protein
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increases enzyme production
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stimulatory effect
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1.9fold stimulation
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stimulation, 137% of non-stimulated activity
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activation
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activation
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activates
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activation
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50 mM, stimulates
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1 mM, relative activity 130%
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best at 2.5% w/v, 28.7% activation
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Inhibitor in Enzyme-catalyzed Reactions (108 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
slight
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9% inhibition at 0.5 mM, 12% inhibition at 5 mM
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at very high concentrations
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inhibits in presence of low amounts of arginine
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hydroxylamine as substrate
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competitive inhibitor of pyruvate-dependent GABA-T activity
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weak, 30% inhibition at 15 mM
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linear competitive inhibitor
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competitive vs. L-serine
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noncompetitive inhibition with respect to delta-aminovalerate, competitive inhibition to 2-oxoglutarate
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20% inhibition at 10 mM
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isoenzyme II, french bean
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weak
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8 mM: 99% of activity compared to H2O
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competitive inhibition
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weak
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competitive against 2-aminoisobutanoate
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the enzymic cleavage of L-threonine stops when about 30% of the amino acid is converted to glycine and acetaldehyde. By dialysis of the incubation medium against buffer the full activity of the enzyme is restored, product inhibition
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competitive
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competitive
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73% inhibition at 4 mM
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (142 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
176.4
-
pH and temperature not specified in the publication
50.08
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apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
5
-
pH 7.5, 70°C
875
-
per active site, pH 8.5, 30°C
3.2
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apparent value, at pH 8.0, temperature not specified in the publication