Ligand angiotensin II

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Basic Ligand Information

Molecular Structure
Picture of angiotensin II (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C50H71N13O12
angiotensin II
CZGUSIXMZVURDU-JZXHSEFVSA-N
Synonyms:
angiotensin-II, Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, DRVYIHPF, Ile5-angiotensin II

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
angiotensin II + H2O = Asp + angiotensin III
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin-II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (7 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (49 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
angiotensin II + H2O = ?
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Ile5-angiotensin II + H2O = ?
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = angiotensin-(1-7) + L-Phe
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = fragments of angiotensin II
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angiotensin II + H2O = hydrolyzed angiotensin II
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angiotensin II + H2O = ?
show the reaction diagram
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angiotensin II + H2O = DRVYIH + Pro-Phe
show the reaction diagram
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angiotensin II + H2O = ?
show the reaction diagram
-
angiotensin II + H2O = ?
show the reaction diagram
-
angiotensin II + H2O = ?
show the reaction diagram
-
angiotensin II + H2O = ?
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
angiotensin I + H2O = DRVYIHPF + His-Leu
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angiotensin I + H2O = angiotensin II + His-Leu
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (20 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
Ang II substantially increases endothelial enzyme protein levels and enzyme-dependent superoxide production in cultured endothelial cells
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induces sEH activity
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1.2-fold increased activity of calpain-1, influence in expression of calpain-1
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activates the enzyme in vivo, reduced by pan-calpain inhibitors
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treatment with angiotensin II results in angiotensin II type 1 receptor AT1R-mediated increase in myocardial TACE expression and activity, and membrane translocation of TACE
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activates
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stimulates Rac1 as cardiovascular stimulus
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angiotension II infusion induces mRNA expression of mPGES-1, mPGES-2 and cytosolic PGE synthase
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Inhibitor in Enzyme-catalyzed Reactions (18 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+
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0.3 mM, 22% inhibition
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inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
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1 microM, about 55% relative activity; 1 microM, about 55% relative activity
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angiotensin II has an activating and regulatory role for Na+-ATPase activity in healthy rats, and angiotensin II links the enzyme activity to hypertension in spontaneously hypertensive rats, where it has an inhibitory effect on Na+-ATPase activity. The Ang II inhibitory effect is completely reversed by a specific antagonist of AT2 receptor, PD123319. Ang II interacts with different receptors in spontaneously hypertensive rats and WKY wild-type rats
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (16 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
87
-
removal of the first dipeptide
1.19
-
-
0.4
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
40
-
37°C, pH 7.4

KM Value (23 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.25
-
-
0.34
-
-
0.191
-
pH 8.1, 37°C
0.036
-
-
0.85
-
-
3.3
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in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.179
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37°C, pH 7.4

Ki Value (6 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
TABLE ID
0.00812
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
31798
0.00795
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
31811

IC50 Value (4 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.065
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+

References & Links

Links to other databases for angiotensin II

ChEBI
PubChem
ChEBI
PubChem