Ligand pyridoxal

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Basic Ligand Information

Molecular Structure
Picture of pyridoxal (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C8H9NO3
pyridoxal
RADKZDMFGJYCBB-UHFFFAOYSA-N


Show all pahtways known for Show all BRENDA pathways known for pyridoxal

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxal + NAD+ = 4-pyridoxolactone + NADH + H+
show the reaction diagram
-
pyridoxal + H2O + O2 = 4-pyridoxate + ?
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxine + NADP+ = pyridoxal + NADPH
show the reaction diagram
-
pyridoxine + O2 = pyridoxal + H2O2
show the reaction diagram
-
-
pyridoxamine + oxaloacetate = pyridoxal + L-aspartate
show the reaction diagram
-
pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (27 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxal + NADPH = 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol + NADP+
show the reaction diagram
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pyridoxal + acceptor = 3-hydroxy-2-methylpyridine-4,5-dicarbaldehyde + reduced acceptor
show the reaction diagram
-
pyridoxal + H2O + O2 = 4-pyridoxic acid + H2O2
show the reaction diagram
-
pyridoxal + H2O + O2 = 4-pyridoxate + ?
show the reaction diagram
-
ATP + pyridoxal = ADP + pyridoxal 5'-phosphate
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (22 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxine + NADP+ = pyridoxal + NADPH
show the reaction diagram
-
pyridoxamine + oxaloacetate = pyridoxal + L-aspartate
show the reaction diagram
-
pyridoxamine + 2-oxoglutarate = pyridoxal + D-glutamate
show the reaction diagram
-
pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate
show the reaction diagram
-
pyridoxal phosphate + H2O = pyridoxal + phosphate
show the reaction diagram
-
pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate
show the reaction diagram
-
-
pyridoxal phosphate + H2O = pyridoxal + phosphate
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
approx. 0.1 mM, 1.4fold increase in activity of isozyme A
-
weak activation
-
is 23% as effective as pyridoxal 5'-phosphate
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Inhibitor in Enzyme-catalyzed Reactions (49 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
substrate inhibition, 90% inhibition at 2 mM
-
less potent than pyridoxal phosphate
-
Ki for 2-amino-4-hydroxypterine oxidation: 0.08 mM at 30 and 50°C, competitive; Ki for xanthine oxidation: 0.05 mM at 30°C, 0.11 mM at 50°C, competitive
-
0.01 mM, 40% inhibition of total enzyme activity in cell extracts
-
NADH and NADPH protect from inactivation
-
phosphorylase I, not II
-
inhibits the activity competitively with respect to pyridoxal 5'-phosphate
-
1 mM, 20% residual activity, competitive
-
weak, 11 mM, 50% inhibition of hydrolysis of pyridoxal 5’-phosphate or pyridoxine 5’-phosphate
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12.5 mM, in presence of 12 mM L-cystine 12% inhibition, in presence of 1 mM L-cystine 37% inhibition
-
2.5 mM, 63% residual activity
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3D Structure of Enzyme-Ligand-Complex (PDB) (8 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (30 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
41
-
; 30°C, pH 9.0

KM Value (45 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.028
-
-

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for pyridoxal

ChEBI
PubChem
ChEBI
PubChem