Ligand 5,10-methylenetetrahydrofolate

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of 5,10-methylenetetrahydrofolate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C20H23N7O6
5,10-methylenetetrahydrofolate
QYNUQALWYRSVHF-OLZOCXBDSA-N
Synonyms:
(-)-5,10-methylene-tetrahydropteroylglutamate, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, (6R)-5,10-methylenetetrahydrofolate, (6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu, 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu, 5,10-methylene-5,6,7,8-tetrahydropteroylmonoglutamate, 5,10-methylene-tetrahydrofolate, 5,10-methylene-tetrahydropteroylglutamate, 5,10-methylene tetrahydrofolate, 5,10-methylenetetrahydropteroylglutamate, 5,10-methylenetetrahydropteroylmonoglutamate, methylenetetrahydropteroylgluamate, methylentetrahydrofolate, N5,10-methylenetetrahydrofolate, N5,N10-methylenetetrahydrofolate, [6R]-methylenetetrahydropteroylglutamate, [6R]methylenetetrahydropteroylglutamate
Pathway Source
Pathways


Show all pahtways known for Show all BRENDA pathways known for 5,10-methylenetetrahydrofolate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (46 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH = glycine + tetrahydrofolate + NAD+
show the reaction diagram
-
5,10-methylenetetrahydrofolate + dCMP = dihydrofolate + deoxy-5-methylcytidylate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
glycine + tetrahydrofolate + NAD+ = 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (103 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH = glycine + tetrahydrofolate + NAD+
show the reaction diagram
-
reduced H-protein + 5,10-methylene-tetrahydrofolate + NH4Cl = ?
show the reaction diagram
-
5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH + H+
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (51 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
glycine + tetrahydrofolate + NAD+ = 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
show the reaction diagram
-
-
5,10-methenyltetrahydrofolate + reduced N,N-dimethylglycine = 5,10-methylene tetrahydrofolate + oxidized N,N-dimethylglycine
show the reaction diagram
-
-
formaldehyde + tetrahydrofolate = methylentetrahydrofolate
show the reaction diagram
-

Enzyme Cofactor/Cosubstrate (13 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (7 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (96 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (23 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.78
-
-

KM Value (95 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0714
-
-
0.5
-
-
0.82
-
-

Ki Value (4 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

Links to other databases for 5,10-methylenetetrahydrofolate

ChEBI
PubChem
-
PubChem