Ligand arsenate

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Basic Ligand Information

Molecular Structure
Picture of arsenate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H3AsO4
arsenate
DJHGAFSJWGLOIV-UHFFFAOYSA-K
Synonyms:
Arsenate ion, arsenic acid, AsO4, AsO43-, HAsO42-


Show all pahtways known for Show all BRENDA pathways known for arsenate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (7 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
arsenite + acceptor = arsenate + reduced acceptor
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (55 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
D-glyceraldehyde 3-phosphate + arsenate + NADP+ = 3-phospho-D-glyceroyl arsenate + NADPH
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pyruvate + arsenate + O2 + H2O = acetyl arsenate + CO2 + H2O2
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arsenate + glutathione = arsenite + reduced glutathione
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arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O
show the reaction diagram
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arsenate + azurin(red) = arsenite + H2O + azurin(ox)
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arsenate + L-citrulline = carbamoylarsenate + L-ornithine
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arsenate + N-carbamoylputrescine = ?
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maltohexaose + arsenate = maltopentaose + glucose 1-arsenate
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cellobiose + arsenate = D-glucose + alpha-D-glucose 1-arsenate
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maltose + arsenate = D-glucose + D-glucose + arsenate
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uric acid ribonucleoside + arsenate = urate + D-ribose 1-phosphate
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S-methyl-5'-thioadenosine + arsenate = adenine + S-methyl-5-thio-alpha-D-ribose 1-arsenate
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uridine + arsenate = ?
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thymidine + arsenate = ?
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arsenate + D-fructose 1,6-bisphosphate = ?
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ATP + pyruvate + arsenate = ?
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ATP + H2O + arsenate/out = ADP + phosphate + arsenate/in
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ATP + H2O + arsenate/in = ADP + phosphate + arsenate/out
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
arsenite + acceptor = arsenate + reduced acceptor
show the reaction diagram
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maltose + arsenate = D-glucose + D-glucose + arsenate
show the reaction diagram
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ATP + H2O + arsenate/out = ADP + phosphate + arsenate/in
show the reaction diagram
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ATP + H2O + arsenate/in = ADP + phosphate + arsenate/out
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (21 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
increasing activity up to 0.1 M, inhibition of ferricyanide-linked assay above 0.1 M
-
activates less than phosphate
-
best at 10 mM and up to 30 mM
-
10 mM increases enzyme activity by 1.6fold
-
absolutely required for protein C activity
-
crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity
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0.8 mM 104% activity
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potent activator in the concentration range of 0-10 mM
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activation, less effective than phosphate
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50 mM, 2.9fold activation of cytosolic PFK
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hyperbolic activation curve, 13% activation at 25 mM, 87% activation at 500 mM
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in absence of reducing agents
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causes further enhancement of enzyme activity when added iin 1-10 mM amounts in the standard assay medium containing KCl. Activity with 12 mM arsenate, is threefold higher than the original aldolase activity
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activates
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increasemM s activity
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activates gamma-glutamyl transferase reaction
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Inhibitor in Enzyme-catalyzed Reactions (73 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
uncompetitive inhibition
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inhibition of glycolate-ferricyanide or glyoxylate-ferricyanide assay above 0.1 M
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competitive inhibitor
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linear substrate inhibition, competitive versus phosphate
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addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme
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complete inhibition of activity in either direction
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100 mM, EPR spectra
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weak
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competitive vs. carbamoylphosphate, noncompetitive vs. ornithine
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competitive vs. carbamoylphosphate
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catalytically active 51 kDa C fragment of hexokinase
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strong competitive inhibitor
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40% inhibition at 1 mM
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10 mM, 10% inhibition
-
competitive. 10 mM, 39% residual activity
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inhibition of protein tyrosine phosphatase activity
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organometallic complexes composed of humic acid and arsenic acid show enhanced inhibition of plasmin activity as compared with either arsenic or humic acid alone
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0.5 M, 74% inhibition
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strong inhibition for formamidase I, slight inhibition for formamidase II at 1 mM
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slight inhibition
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inhibition of 5-aminolevulinic acid dehydratase activity by arsenic in excised etiolated maize leaf segments during greening. KNO3, chloramphenical, cycloheximide, DTNB and levulinic aciddecrease inhibition. GSH increase inhibition
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1 mM, 92% of initial activity
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Metals and Ions (11 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation
-
or phosphate is required
-
stimulates at 10 mM, 1 mM and 0.1 mM
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50 mM, 800% of the relative activity without addition
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activates
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increases storage stability
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3D Structure of Enzyme-Ligand-Complex (PDB) (3 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (45 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
14400
-
-
4.9
-
maltohexaose arsenolysis
1.15
-
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KM Value (73 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
1.25
-
electron acceptor: oxygen
109
-
-
0.3
-
-
0.793
-
-
4
-
arsenolytic cleavage
22.7
-
maltohexaose arsenolysis
1.7
-
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Ki Value (32 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.12
-
-
93
-
pH 8.0, temperature not specified in the publication, recombinant His-tagged wild-type enzyme
12
-
-
0.6
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pH 3.5, 37°C

References & Links

Links to other databases for arsenate

ChEBI
PubChem
ChEBI
PubChem