Ligand reduced flavodoxin

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Basic Ligand Information

Molecular Structure
Picture of reduced flavodoxin (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H11N4O2R
reduced flavodoxin
GQPPNUDTGJSKSD-UHFFFAOYSA-N
Synonyms:
4a,5-reduced flavin, dihydroflavodoxin, flavodoxin semiquinone, reduced electron-transfer flavoprotein, reduced electron-transferring-flavoprotein, reduced electron-transferring flavoprotein, reduced electron transfer flavoprotein, reduced electron transferring flavoprotein, reduced flavin, reduced flavoprotein

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
a long-chain acyl-[acyl-carrier protein] + reduced flavodoxin + O2 = pimeloyl-[acyl-carrier protein] + an n-alkanal + oxidized flavodoxin + H2O
show the reaction diagram
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
show the reaction diagram
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crotonobetainyl-CoA + reduced electron-transfer flavoprotein = gamma-butyrobetainyl-CoA + electron-transfer flavoprotein
show the reaction diagram
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nitrate + reduced flavodoxin = nitrite + oxidized flavodoxin + H2O
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2 S-adenosyl-L-methionine + protoheme + 2 reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + anaerobilin + Fe2+ + 2 oxidized flavodoxin
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (25 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
show the reaction diagram
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(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein = 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
show the reaction diagram
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isovaleryl-CoA + electron-transfer flavoprotein = 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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2-methylbutanoyl-CoA + electron transfer flavoprotein = 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
show the reaction diagram
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NADH + flavin = NAD+ + reduced flavin
show the reaction diagram
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sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (79 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
16alpha-hydroxytestosterone + 3 O2 + 3 reduced flavoprotein = (16alpha,17beta)-estra-1(10),2,4-triene-3,16,17-triol + formate + 4 H2O + 3 oxidized flavoprotein
show the reaction diagram
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11-deoxycorticosterone + reduced flavodoxin + O2 = 15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O
show the reaction diagram
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
show the reaction diagram
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6 reduced flavodoxin + N2 + 6 H2O + 6 ATP = 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
show the reaction diagram
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reduced flavodoxin + NADP+ = oxidized flavodoxin + NADPH + H+
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crotonobetainyl-CoA + reduced electron-transfer flavoprotein = gamma-butyrobetainyl-CoA + electron-transfer flavoprotein
show the reaction diagram
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reduced flavin + NAD+ = flavin + NADH + H+
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reduced flavin + nitrate = ? + nitrite
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2 S-adenosyl-L-methionine + menaquinone-6 + reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methylmenaquinone-6 + oxidized flavodoxin
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (88 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
7-methoxy-4-trifluoromethyl coumarin + H2O + oxidized flavoprotein = ? + O2 + reduced flavoprotein
show the reaction diagram
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(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
show the reaction diagram
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(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein = 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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gamma-butyrobetainyl-CoA + electron-transfer flavoprotein = crotonobetainyl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
show the reaction diagram
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palmitoyl-CoA + electron-transfer flavoprotein = (2E)-2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
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2-methylbutanoyl-CoA + electron transfer flavoprotein = 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
show the reaction diagram
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NADH + flavin = NAD+ + reduced flavin
show the reaction diagram
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sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
under anaerobic growth conditions, oxidized ferredoxin (flavodoxin):NADP+ oxidoreductase accepts a hydride from NADPH and transfers the electron to flavodoxin, generating primarily flavodoxin semiquinone. Under anaerobic conditions the decarboxylation of pyruvate is coupled to reduction of flavodoxin, forming the flavodoxin hydroquinone. These reduced forms of flavodoxin bind to inactive cob(II)alamin enzyme, leading to a conformational change that is coupled with dissociation of His759 and protonation of the His759-Asp757-Ser810 triad. Although NADPH oxidation ultimately produces 2 equivalent of flavodoxin semiquinone, only one electron is transferred to methionine synthase during reductive methylation
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a better electron donor than reduced ferredoxin
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chromophore, enzyme I
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (10 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.25
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pH 8.0

KM Value (6 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for reduced flavodoxin

ChEBI
PubChem
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PubChem