Ligand 2',5'-ADP

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Basic Ligand Information

Molecular Structure
Picture of 2',5'-ADP (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H15N5O10P2
2',5'-ADP
AEOBEOJCBAYXBA-KQYNXXCUSA-N
Synonyms:
2',5'-adenosine bisphosphate, 2',5'-Adenosine diphosphate, 2'-phospho-AMP, adenosine 2',5'-bisphosphate, adenosine 2',5'-phosphate

Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
adenosine 2',5'-bisphosphate + 4-nitrophenyl sulfate = adenosine 2'-phosphate 5'-phosphate 5'-sulfate + 4-nitrophenol
show the reaction diagram
-
2',5'-ADP + H2O = ?
show the reaction diagram
-
adenosine 2',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
increases internal electron transfer in dithionite reduced enzyme
-

Inhibitor in Enzyme-catalyzed Reactions (37 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive
-
NADP+-competitive and NAD+-noncompetitive inhibition
-
2',5'-ADP inhibits the NADPH-ferricyanide reductase activity of the enzyme competitively
-
competitive inhibition of NADPH binding
-
act as a competitive ihibitor
-
bacteriorhodopsin co-reconstituted enzyme, 36.8% inhibition of thio-NADP+ reduction by NADH in the dark, 34.4% in the light
-
millimolar concentrations
-
44% inhibition at concentration of 0.133 mM
-
poly(A) cyclization
-

3D Structure of Enzyme-Ligand-Complex (PDB) (15 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
13.2
-
in 10 mM HEPES pH 7.0

KM Value (3 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.067
-
pH 7.0, 25°C

Ki Value (22 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.00134
-
pH 7.8, 23°C
0.24
-
pH 7.6
0.12
-
pH 7.8, 25°C, substrate: NADP+
0.28
-
at 25°C in 50 mM potassium phosphate, pH 7.4
0.00302
-
calculated competitive inhibition constant
0.106
-
-

References & Links

Links to other databases for 2',5'-ADP

ChEBI
PubChem
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PubChem