Ligand cystatin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Formula
BRENDA Name
InChIKey
cystatin

Roles as Enzyme Ligand

Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates
-

Inhibitor in Enzyme-catalyzed Reactions (40 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
hybrids of chicken cystatin with human kininogen domain 2 sequences exhibit improved inhibition
-
from chicken
-
chicken, weak inhibition
-
different types, expression analysis in various cell types isolated from normal and neoplastic breast tissue, overview
-
engineered cystatins. Recombinant hybrids of human stefin B with KS2 and DELTAL110 deletion mutants of chicken cystatin-KD2 hybrids. Substitution of the N-terminal contact region of stefin B by ther corresponding KD2 sequence results in a calpain inhibitor with a Ki-value of 188 nM. Deletion of L110 improves inhibition 4 to 8fold. All engineered cystatins are temporary inhibitors
-
reversible inhibition
-
47.5% inhibition at 0.05 mM
-
1 microM, 37°C, 48 h, pH 5.0
-
0.0133 mg/mL 46.38% inhibition
-

Enzyme Kinetic Parameters

Ki Value (2 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.000029
-
-
0.00003
-
26°C, pH 6.0

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.06
-
at pH 5.0 and 37°C

References & Links

Links to other databases for cystatin