Ligand HgCl2

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Basic Ligand Information

Molecular Structure
Picture of HgCl2 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2Cl2Hg
HgCl2
LWJROJCJINYWOX-UHFFFAOYSA-L
Synonyms:
Mercury chloride

Roles as Enzyme Ligand

Activator in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.01 mM, 3.4fold increase in activity, exo-PG2, no effect with exo-PG1
-

Inhibitor in Enzyme-catalyzed Reactions (584 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
2.5 mM, 94% inhibition
-
complete inhibition at 2 mM
-
at 1 mM 94-100% inhibition, depending on the substrate; IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
-
complete inactivation at 0.5 mM
-
complete inhibition at concentration 0.05 mM
-
1 mM, complete inhibition
-
1 mM, no residual activity
-
0.1 mM, complete inhibition
-
1 mM, complete inhibition
-
0.02 mM, 67% loss of activity after 1 h
-
strong
-
1 mM, 100% inhibition
-
activity of the oxidized enzyme in presence of guanidine is unaffected by 0.1 mM HgCl2 in presence of 1 mM EDTA and by 0.005 mM HgCl2 in absence of EDTA, activity of the reduced form of enzyme is completely abolished
-
complete inhibition
-
2 mM, no residual activity
-
1 mM, 44% inhibition
-
above 0.001 mM
-
28% inhibition at 1 mM
-
30.8% inhibition at 1 mM
-
68% inhibition at 0.05 mM, complete inhibition at 1 mM
-
1 mM, 92% inhibition
-
1 mM causes 100% inhibition
-
complete
-
10 mM, 100% inhibition
-
1 mM, 93% loss of activity
-
84% inhibition at 1 mM
-
0.001 mM, complete inhibition
-
20 mM, complete inhibition
-
12% residual activity at 0.5 mM
-
1 mM, 85% inhibition
-
1 mM, complete inhibition
-
92% inactivation at 0.1 mM
-
0.01 mM, complete inhibition
-
0.01 mM
-
IC50: 4.7 mM, liver microsomes
-
complete inhibition at 0.1 mM
-
0.2 mM, 50% inhibition
-
1 mM, inhibition of isozyme SODI
-
activity is inhibited by an excess of HgCl2
-
complete inhibition at 1 mM
-
1 mM, 10 min at 35°C, complete loss of activity
-
at 0.5 mM complete inhibition of enzyme 1 and enzyme 2
-
1 mM 31% inhibition
-
0.02 mM, 67% loss of activity after 1 h
-
0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibtion at 1 mM
-
0.1 mM 36% inhibition
-
1 mM, cosubstate ferricyanide, strong inhibition; 1 mM, strong inhibition
-
0.1 mM, complete loss of activity, most potent inhibitor
-
irreversible inhibition
-
3 mM: more than 99% inhibition, inhibition negligible in the presence of 10 mM glutathione
-
0.1 mM, complete inhibition
-
1 mM, complete inhibition
-
at 0.001 mM remains 8% of activity
-
0.1 mM, 91% inhibition
-
1 mM, strongly inhibited,more than 90%
-
0.1 mM, 71% inhibition
-
1 mM, 75% inhibition
-
0.01 mM, 89% loss of activity
-
0.1-1 mM
-
100% inhibition at 0.005 mM
-
100% inhibition at 1 mM
-
0.5 mM, 80% inhibition
-
0.02 mM
-
0.2 mM, 94% inhibition
-
10 mM, complete inhibition
-
0.002 mM, 92% inhibition
-
1 mM, 90% inhibition
-
0.001 mM, 50% inhibition
-
0.1 mM, complete inhibition
-
90% at 0.03-0.05 mM, reversible by DTT
-
strong
-
5 mM, 96% inhibition in the presence of 10 mM Ca2+
-
immobilized enzyme: 54% inhibition at 0.1 mM, 61% inhibition at 1 mM, 79% inhibition at 10 mM. Free enzyme: 61% inhibition at 0.1 mM, 75% inhibition at 1 mM, 91% inhibition at 10 mM
-
0.1 mM, 85% inhibition
-
0.1 mM, 95% inhibition, irreversible inhibition
-
strong
-
strong
-
0.3 mM, complete inhibition
-
0.1 mM, complete loss of activity
-
10% inhibition at 1 mM
-
strong
-
strong
-
slight inhibition of mutant, not wild-type
-
potent inhibitor at 0.001 mM
-
at 0.01 mM 85% inhibition, at 0.1 mM complete inhibition
-
25°C, 10 min, 98.3% inhibition of the O-phospho-L-serine sulfhydrylation reaction, 80% inhibition of the O-acetyl-L-serine sulfhydrylation reaction
-
0.1 mM, complete inhibition
-
1 mM, 100% inhibition
-
strong inhibition
-
irreversible and complete inhibition is observed at concentrations about 0.1 mM
-
40% activity retained at 1 mM, 31% activity retained at 10 mM
-
2 mM, 32% inhibition
-
strong
-
0.1 mM, 90% inhibition
-
5 mM, complete inhibition
-
0.2 mM, 51% inhibition
-
10 mM, 47% inhibition
-
weak
-
10 mM, complete inhibition
-
94% inhibition at 0.2 mM
-
inhibition is reversed by incubation with an excess amount of dithiothreitol and 2-mercaptoethanol
-
0.1 mM, 50% inhibition
-
1 mM, complete inhibition
-
92% inhibition at 5 mM
-
complete inhibition at 1 mM, 80% inhibition at 0.1 mM
-
strong inhibition
-
99% inhibition at 1 mM; 99% inhibition at 1 mM
-
100% inhibition at 0.2 mM
-
0.1 mM 95% inhibition
-
the enzyme is relatively insensitive to sulfhydryl reagents, increased inhibition after addition of dithiothreitol
-
10 mM, 83% inhibition
-
2 mM, 92% inhibition
-
weak
-
0.05 mM, complete loss of activity
-
3 mM, 98.6% inhibition
-
1 mM, 73% activity compared to control without any metal ion
-
1 mM, complete inhibition
-
2 mM, no residual activity
-
10 mM, complete inhibition
-
1 mM, 96% loss of activity
-
0.001 M, 80% loss of activity
-
0.001 M, 80% loss of activity
-
0.0022 mM 50% inactivation
-
10 mM, complete inhibition
-
at 1 mM causes 17% inhibition of PGI, at 5 mM causes 48% inhibition of PGI
-
2 mM, complete inhibition
-
2 mM, about 95% loss of activity
-
strong inhibition at 1 mM
-
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
-
5 mM, 98% inhibition in presence of 1 mM CoCl2
-
0.5 mM, relative activity: 31%
-
complete inhibition at 1 mM
-
30°C, 10 min, 1 mM, 99% loss of activity
-
IC50: 0.5 mM
-
80% inhibition at 10 mM
-
slight
-
slight
-
inhibition of prekallikrein activation
-
99% inhibition at 1 mM
-
complete inhibition at 0.05 mM
-
reversible by 2-mercaptoethanol
-
49% inhibition at 0.5 mM, 60% at 5 mM
-
complete inhibition
-
1 mM, 73% inhibition, addition of 10 mM dithiothreitol restores activity to 100%
-
1 mM, incubation at 30°C for 10 min, complete inhibition
-
1 mM, inhibition reversed by 2-mercaptoethanol
-
1 mM, 100% inhibition
-
43.2% residual activity
-
1 mM: strong inhibition
-
0.005 mM: 100% inhibition
-
1 mM, complete loss of activity
-
99% inibition at 1 mM
-
1 mM, dextran-conjugated enzyme and native enzyme retain 40% and 13.4% of their initial activities
-
69% inhibition in 5 min
-
inhibition of ADA activity is observed in the soluble fraction at 0.005-0.25 mM HgCl2 (84.6-92.6% respectively), whereas inhibition occurs at 0.005-0.25 mM in membrane fractions (20.9-26% respectively). The inhibition is partially or fully abolished by 0.5 mM dithiothreitol or EDTA
-
complete inhibition
-
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
-
Ki: 0.6 mM
-
2.5 mM, 78% loss of activity
-
1 mM, complete inhibition of decarboxylation activity, 94% loss of carboxylation activity
-
1 mM, 69% inhibition
-
1 mM, complete inhibition
-
1 mM, 100% inhibition
-
1 mM, complete inhibition
-
reversible
-
1 mM, 99% inhibition
-
0.1 mM, 41% inhibition
-
1 mM, 99% inhibition
-
reduces enzyme activity by 40% at 0.01 mM, 80% at 0.1 mM, and 98% at 0.2 mM
-
pretreatment with a nontoxic dose of Na2SeO3 partially or totally prevents in vivo mercury effects in kidney, including prevention of inhibition of delta-aminolevulinate dehydratase
-
0.25 mM, ca. 90% inhibition
-
89% reduced activity at 1 mM
-
; 0.1 mM, 87% inhibition
-
1 mM, 23% remaining activity
-
1 mM, complete inhibition
-
complete inactivation at 0.1 mM
-
1 mM, 89.5% inhibition
-
0.05 mM, triethanolamine-HCl, pH 8.0, 95% loss of activity
-
a potent irreversible enzyme inhibitor, but HgCl2 is also a strong inactivator of cystathionine gamma-lyase
-
57% inhibition at 1 mM
-
67% loss in Gly-I activity by 0.03 mM HgCl2, Gly-I inactivation by HgCl2 is fully prevented or reversed by 0.5 mM N-acetylcysteine
-
inhibitory at 100microM
-
21.25% residual activity at 10 mM
-
0.1 M, complete inhibition, pH 7.0, 30°C, 30 min
-
0.1 mM, 100% inhibition of D-erythrose 4-phosphate isomerase activity
-
1 mM, 80% inhibition
-
dose-dependent inhibition of forskolin/3-isobutyl-1-methylxanthine-stimulated chloride secretion, inhibition is maximal when HgCl2 is added before stimulation with forskolin/3-isobutyl-1-methylxanthine, dithiothrietol and glutathione completely prevent inhibition of chloride secretion, inhibits chloride secretion by interacting with the apical membrane
-
uncompetitive, 50 nM, 15% inhibition, 400 nM, 80% inhibition, DTT or glutathione protect, full activity is restored by 500 nM DTT or glutathione
-
-
-

Metals and Ions (16 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1 mM, 1% residual activity
-
0.25 mM, activation to 137% of control
-
1 mM, 68% loss of activity
-
at 0.1 M and 0.3 mM, no influence on the activity of the pSCS6-encoded enzyme. The activity of the pSCS7-encoded enzyme decreases to 10-15%. At 1.0 mM HgCl2, the pSCS6-encoded enzyme retains 50% of ist original activity, complete inactivation of the enzyme from pSCS7-encoded enzyme
-
5 mM, free enzyme: 13.7% inhibition, immobilized enzyme: 30% inhibition
-
activates
-
10 mM, stimulates
-
0.1 mM, activation to 130% of control
-
1 mM, 13% inhibition
-
1 mM, 73% activity compared to control without any metal ion
-
1 mM, complete inhibition, isoenzyme MpiCP-2
-
activates
-
decreased activity
-
5 mM, complete inhibition
-
relative activity: 29%
-
a potent irreversible enzyme inhibitor, but HgCl2 is also a strong inactivator of cystathionine gamma-lyase
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
12.4
-
-

KM Value (5 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (7 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
TABLE ID
0.3
-
prostaglandin D2 11-ketoreductase activity
1162
0.2
-
-
33099
0.6
-
-
34689
0.28
-
pH 9.5, 30°C
34919
0.0002
-
pH 7.0, 30°C
41427

IC50 Value (4 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.3
-
IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
4.7
-
IC50: 4.7 mM, liver microsomes
0.042
-
IC50 value is 0.042 mM
0.5
-
IC50: 0.5 mM

References & Links

Links to other databases for HgCl2

ChEBI
PubChem
ChEBI
PubChem