Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/in
-
ABC transporter, HMT-1, confers tolerance to cadmium. HMT-1 is not essential for vacuolar phytochelatin sequestration. HMT-1 either does not transport Cd-PC complexes or is not the principal Cd-PC/apoPC transporter
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
ATP + H2O + Co2+[side 1]
ADP + phosphate + Co2+[side 2]
-
-
-
?
ATP + H2O + estradiol-beta-17-glucuronide [side 1]
ADP + phosphate + estradiol-beta-17-glucuronide [side 2]
-
-
-
-
?
ATP + H2O + leukotriene C4 [side 1]
ADP + phosphate + leukotriene C4 [side 2]
-
-
-
-
?
ATP + H2O + Zn2+[side 1]
ADP + phosphate + Zn2+[side 2]
lowest activity
-
-
?
bilirubin/cytoplasm + ATP + H2O
bilirubin/vacuole + ADP + phosphate
-
unconjugated substrate
-
-
?
Cd x glutathione S2/cytoplasm + ATP + H2O
Cd x glutathione S2/vacuole + phosphate
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
Cd2+/cytoplasm + ATP + H2O
Cd2+/vacuole + ADP + phosphate
-
-
-
-
?
diazaborine/cytoplasm + ATP + H2O
diazaborine/vacuole + ADP + phosphate
-
-
-
-
?
glutathione S-conjugated leukotriene C4/cytoplasm + ATP + H2O
glutathione S-conjugated leukotriene C4/vacuole + ADP + phosphate
-
-
-
-
?
glutathione S-conjugates/cytoplasm + ATP + H2O
glutathione S-conjugates/vacuole + ADP + phosphate
-
-
-
-
?
glutathione/cytoplasm + ATP + H2O
glutathione/vacuole + ADP + phosphate
-
-
-
-
?
S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O
S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
-
-
-
?
additional information
?
-
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
Q0WLU3
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
Q0WLU3
chloroplast envelope membrane protein OSA1, a member of the Abc1-like family, acts as a factor in cadmium and oxidative stress response, playing a role in the balance of oxidative stress
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ABC transporter, DmHMT-1, confers tolerance to cadmium. DmHMT-1 and its yeast homolog, SpHMT-1, are not essential for vacuolar phytochelatin sequestration
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
transports glutathione-S-conjugates, like Cd-GS2, from the cytosol into the vacuole
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Cd2+ is a toxic environmental contaminant for biological systems, which can form complexes with reduced glutathione, and thus alter the intracellular redox state. In Saccharomyces cerevisiae, bis(glutathionato)cadmium, Cd-[GS]2, complexes can be removed from the cytosol and transported into the vacuole by a glutathione-conjugated pump, Ycf1p, which plays a role in Cd2+ detoxi?cation during respiratory metabolism and is correlated with the glutathione homeostasis, overview
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
the yeast ABC transporter belongs to the ABCC subfamily of ATP-binding cassette, ABC, transporters that rid cells of toxic endogenous and xenobiotic compounds, negative regulation of Ycf1p by phosphorylation within its N-terminal extension in addition to its ABC core domain and transports substrates in the form of glutathione conjugates
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 is a cadmium transporter, the N-terminal metal-responding degradation signal encompassing amino acids 250-350 functions autonomously in a metal-responsive manner, overview
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Ycf1p-dependent transport of [3H]estradiol-beta-17-glucuronide in vitro
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Pca1 is a cadmium transporter, the N-terminal metal-responding degradation signal encompassing amino acids 250-350 functions autonomously in a metal-responsive manner, overview
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Cd2+ is a toxic environmental contaminant for biological systems, which can form complexes with reduced glutathione, and thus alter the intracellular redox state. In Saccharomyces cerevisiae, bis(glutathionato)cadmium, Cd-[GS]2, complexes can be removed from the cytosol and transported into the vacuole by a glutathione-conjugated pump, Ycf1p, which plays a role in Cd2+ detoxi?cation during respiratory metabolism and is correlated with the glutathione homeostasis, overview
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
highest activity
-
-
?
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
-
-
-
-
?
additional information
?
-
Q0WLU3
in the absence of Cd21, an increase in the expression of AtOSA1 is correlated with plant aging. The transcript level of AtOSA1, which is age dependent, is also down-regulated in the night, circadian rhythm dependencies, and senescent leaves
-
-
?
additional information
?
-
-
Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases
-
-
?
additional information
?
-
enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains
-
-
?
additional information
?
-
-
enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains
-
-
?
additional information
?
-
-
Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/in
-
ABC transporter, HMT-1, confers tolerance to cadmium. HMT-1 is not essential for vacuolar phytochelatin sequestration. HMT-1 either does not transport Cd-PC complexes or is not the principal Cd-PC/apoPC transporter
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
ATP + H2O + Co2+[side 1]
ADP + phosphate + Co2+[side 2]
-
-
-
?
ATP + H2O + Zn2+[side 1]
ADP + phosphate + Zn2+[side 2]
lowest activity
-
-
?
bilirubin/cytoplasm + ATP + H2O
bilirubin/vacuole + ADP + phosphate
-
unconjugated substrate
-
-
?
Cd x glutathione S2/cytoplasm + ATP + H2O
Cd x glutathione S2/vacuole + phosphate
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
diazaborine/cytoplasm + ATP + H2O
diazaborine/vacuole + ADP + phosphate
-
-
-
-
?
glutathione S-conjugated leukotriene C4/cytoplasm + ATP + H2O
glutathione S-conjugated leukotriene C4/vacuole + ADP + phosphate
-
-
-
-
?
glutathione S-conjugates/cytoplasm + ATP + H2O
glutathione S-conjugates/vacuole + ADP + phosphate
-
-
-
-
?
glutathione/cytoplasm + ATP + H2O
glutathione/vacuole + ADP + phosphate
-
-
-
-
?
S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O
S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
-
-
-
?
additional information
?
-
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
Q0WLU3
chloroplast envelope membrane protein OSA1, a member of the Abc1-like family, acts as a factor in cadmium and oxidative stress response, playing a role in the balance of oxidative stress
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
ABC transporter, DmHMT-1, confers tolerance to cadmium. DmHMT-1 and its yeast homolog, SpHMT-1, are not essential for vacuolar phytochelatin sequestration
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
-
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
transports glutathione-S-conjugates, like Cd-GS2, from the cytosol into the vacuole
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Cd2+ is a toxic environmental contaminant for biological systems, which can form complexes with reduced glutathione, and thus alter the intracellular redox state. In Saccharomyces cerevisiae, bis(glutathionato)cadmium, Cd-[GS]2, complexes can be removed from the cytosol and transported into the vacuole by a glutathione-conjugated pump, Ycf1p, which plays a role in Cd2+ detoxi?cation during respiratory metabolism and is correlated with the glutathione homeostasis, overview
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
the yeast ABC transporter belongs to the ABCC subfamily of ATP-binding cassette, ABC, transporters that rid cells of toxic endogenous and xenobiotic compounds, negative regulation of Ycf1p by phosphorylation within its N-terminal extension in addition to its ABC core domain and transports substrates in the form of glutathione conjugates
-
-
?
ATP + H2O + Cd2+/in
ADP + phosphate + Cd2+/out
-
Cd2+ is a toxic environmental contaminant for biological systems, which can form complexes with reduced glutathione, and thus alter the intracellular redox state. In Saccharomyces cerevisiae, bis(glutathionato)cadmium, Cd-[GS]2, complexes can be removed from the cytosol and transported into the vacuole by a glutathione-conjugated pump, Ycf1p, which plays a role in Cd2+ detoxi?cation during respiratory metabolism and is correlated with the glutathione homeostasis, overview
-
-
?
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
highest activity
-
-
?
ATP + H2O + Cd2+[side 1]
ADP + phosphate + Cd2+[side 2]
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
-
-
-
-
?
Cd/cytoplasm + ATP + H2O
Cd/vacuole + ADP + phosphate
-
-
-
-
?
additional information
?
-
Q0WLU3
in the absence of Cd21, an increase in the expression of AtOSA1 is correlated with plant aging. The transcript level of AtOSA1, which is age dependent, is also down-regulated in the night, circadian rhythm dependencies, and senescent leaves
-
-
?
additional information
?
-
-
Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases
-
-
?
additional information
?
-
enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains
-
-
?
additional information
?
-
-
enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains
-
-
?
additional information
?
-
-
Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
YOR1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1477
11
166728
Swiss-Prot
other Location (Reliability: 1)
YCFI_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1515
14
171121
Swiss-Prot
other Location (Reliability: 5)
A0A2G9I694_9LAMI
699
0
78505
TrEMBL
Mitochondrion (Reliability: 4)
A0A072VE85_MEDTR
654
0
73445
TrEMBL
Chloroplast (Reliability: 2)
A0A2P6QNV4_ROSCH
794
0
87364
TrEMBL
Chloroplast (Reliability: 1)
A0A2P6PY58_ROSCH
742
0
84109
TrEMBL
Mitochondrion (Reliability: 5)
M4YXM5_STREQ
631
6
68882
TrEMBL
-
A0A2G9GT33_9LAMI
673
0
74656
TrEMBL
Chloroplast (Reliability: 1)
A0A2G9HCT4_9LAMI
563
0
63647
TrEMBL
Mitochondrion (Reliability: 5)
A0A072UEL7_MEDTR
716
5
78488
TrEMBL
Chloroplast (Reliability: 2)
A0A2P6RR67_ROSCH
754
1
85295
TrEMBL
Chloroplast (Reliability: 2)
A0A2G9FWI1_9LAMI
697
1
78771
TrEMBL
Chloroplast (Reliability: 4)
C5WHV3_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
620
6
67493
TrEMBL
-
G7JA88_MEDTR
744
1
83729
TrEMBL
Chloroplast (Reliability: 3)
A0A072VP08_MEDTR
623
0
68074
TrEMBL
other Location (Reliability: 3)
A0A8B6J266_STRPY
620
7
67672
TrEMBL
-
A0A2G9GQG0_9LAMI
481
0
54548
TrEMBL
other Location (Reliability: 4)
A0A2P6QBF3_ROSCH
583
1
66243
TrEMBL
Mitochondrion (Reliability: 2)
A0A448DHH9_STREQ
620
5
67257
TrEMBL
-
A0A396JKB4_MEDTR
1031
0
114164
TrEMBL
Secretory Pathway (Reliability: 1)
G7J8V4_MEDTR
708
2
80449
TrEMBL
Chloroplast (Reliability: 2)
G7KD96_MEDTR
534
0
59564
TrEMBL
Chloroplast (Reliability: 5)
A0A8H2N311_STRPY
620
7
67776
TrEMBL
-
A0A2P6PZP5_ROSCH
679
0
76211
TrEMBL
Chloroplast (Reliability: 3)
A0A2G9GM21_9LAMI
469
1
53905
TrEMBL
Secretory Pathway (Reliability: 3)
A0A396ICV4_MEDTR
478
0
54139
TrEMBL
other Location (Reliability: 3)
A0A396IRR9_MEDTR
545
0
61666
TrEMBL
Secretory Pathway (Reliability: 4)
G7I9J4_MEDTR
824
0
92243
TrEMBL
Chloroplast (Reliability: 3)
A0A2P6SJJ0_ROSCH
833
0
93766
TrEMBL
Chloroplast (Reliability: 4)
A0A2P6Q3H9_ROSCH
635
1
72483
TrEMBL
Mitochondrion (Reliability: 4)
A0A2P6PRH6_ROSCH
709
0
79324
TrEMBL
Chloroplast (Reliability: 3)
A0A2G9G641_9LAMI
630
0
71359
TrEMBL
Mitochondrion (Reliability: 2)
A0A380JVF6_STRDY
620
5
67370
TrEMBL
-
A0A8H2RE51_STRCB
620
7
67779
TrEMBL
-
A0A396HWG3_MEDTR
535
0
61681
TrEMBL
Chloroplast (Reliability: 3)
A0A2G9GQB6_9LAMI
800
1
89886
TrEMBL
Chloroplast (Reliability: 1)
A0A8B6L6I6_STREQ
620
5
67323
TrEMBL
-
A0A2G9HD84_9LAMI
790
0
87269
TrEMBL
Chloroplast (Reliability: 4)
A0A2P6SLT6_ROSCH
551
0
61270
TrEMBL
Chloroplast (Reliability: 5)
A0A2P6PZW3_ROSCH
1314
0
144921
TrEMBL
Mitochondrion (Reliability: 4)
A0A2G9HSD8_9LAMI
712
2
81060
TrEMBL
Chloroplast (Reliability: 3)
A0A072THI5_MEDTR
701
0
78174
TrEMBL
Chloroplast (Reliability: 2)
G7K5W0_MEDTR
718
5
78725
TrEMBL
Mitochondrion (Reliability: 3)
G7JFX0_MEDTR
713
0
79878
TrEMBL
Chloroplast (Reliability: 1)
A0A2G9H8W8_9LAMI
724
1
81256
TrEMBL
Mitochondrion (Reliability: 2)
A0A2P6P554_ROSCH
482
0
54605
TrEMBL
other Location (Reliability: 5)
A0A2G9HCZ0_9LAMI
540
0
61111
TrEMBL
other Location (Reliability: 3)
A0A396IG19_MEDTR
543
6
60550
TrEMBL
other Location (Reliability: 3)
A0A072TQ18_MEDTR
622
1
70804
TrEMBL
Mitochondrion (Reliability: 2)
A0A2P6S1B4_ROSCH
713
1
80416
TrEMBL
Chloroplast (Reliability: 2)
A0A2P6QNX6_ROSCH
624
1
71155
TrEMBL
Mitochondrion (Reliability: 4)
A0A396IXW0_MEDTR
713
0
80969
TrEMBL
Mitochondrion (Reliability: 3)
A0A2G9G850_9LAMI
537
0
60261
TrEMBL
Mitochondrion (Reliability: 4)
A0A2G9H3C7_9LAMI
160
0
18590
TrEMBL
other Location (Reliability: 3)
Q2HTV1_MEDTR
630
1
71085
TrEMBL
Mitochondrion (Reliability: 5)
A0A2G9GMV1_9LAMI
539
0
60902
TrEMBL
Mitochondrion (Reliability: 5)
A0A509D9T6_9STRE
620
5
67263
TrEMBL
-
A0A2G9HTI2_9LAMI
732
5
80759
TrEMBL
Chloroplast (Reliability: 3)
A0A2G9GMS4_9LAMI
478
1
53562
TrEMBL
Secretory Pathway (Reliability: 5)
A0A239ST63_9STRE
607
5
65801
TrEMBL
-
G7LB96_MEDTR
735
1
83051
TrEMBL
Chloroplast (Reliability: 2)
A0A072UA79_MEDTR
710
0
79370
TrEMBL
Chloroplast (Reliability: 2)
A0A2P6RG05_ROSCH
532
0
60299
TrEMBL
other Location (Reliability: 5)
A0A2P6RF11_ROSCH
611
0
66636
TrEMBL
other Location (Reliability: 3)
A0A2P6SL87_ROSCH
539
1
61117
TrEMBL
Secretory Pathway (Reliability: 5)
A0A2G9GVX7_9LAMI
626
1
70895
TrEMBL
Mitochondrion (Reliability: 3)
A0A2G9GCN2_9LAMI
732
5
80732
TrEMBL
Chloroplast (Reliability: 3)
A0A072VGN0_MEDTR
784
0
86552
TrEMBL
Chloroplast (Reliability: 2)
A0A2P6QL25_ROSCH
718
5
79320
TrEMBL
Mitochondrion (Reliability: 4)
A0A380KCR8_STREQ
620
6
67523
TrEMBL
-
A0A396J1D9_MEDTR
624
1
71082
TrEMBL
Mitochondrion (Reliability: 3)
A0A2P6PC68_ROSCH
720
0
79782
TrEMBL
Chloroplast (Reliability: 3)
A0A2G9H0P7_9LAMI
800
1
89884
TrEMBL
Chloroplast (Reliability: 2)
A0A2Z6G352_STRDY
620
5
67271
TrEMBL
-
HMT1_CAEEL
801
0
90575
Swiss-Prot
-
Q1LAJ7_CUPMC
Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)
829
0
86994
TrEMBL
-
HMA3_ORYSJ
1004
5
102664
Swiss-Prot
-
Q9VF20_DROME
866
10
97886
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C476A
the mutant enzyme binds 0.61 mol equiv of Co2+. The mutant enzyme shows about 4%, about 24%, and about 30% of Cd2+-, Co2+-, and Zn2+-stimulated ATP hydrolysis activity, respectively, compared to the wild type enzyme
H807A
the mutant enzyme retains the ability to bind about 1 mol equiv of all metal ions (Co2+, Zn2+, and Cd2+). The mutation results in a strong decrease in Co2+- (about 17% of wild type) and Zn2+-stimulated (about 16% of wild type) ATP hydrolysis
M254A
the mutant enzyme binds 0.34 mol and 0.26 mol equiv of Cd2+ and Co2+, respectively. The mutation dramatically reduces Cd2+- and Co2+-stimulated ATPase activity (about 8% and about 32% of wild type activity, respectively), whereas Zn2+-stimulated ATPase activity remains virtually unchanged
S474A
the mutant enzyme binds 0.45 mol equiv of Co2+. The mutation modestly decreases Co2+-stimulated ATP hydrolysis (about 66% of wild type activity) whereas Cd2+- and Zn2+-stimulated ATP hydrolysis remain virtually unchanged
S474A/C476A
the mutant enzyme shows about 2%, about 16%, and about 20% of Cd2+-, Co2+-, and Zn2+-stimulated ATP hydrolysis activity, respectively, compared to the wild type enzyme
D398A
-
non-phosphorylatable protein, no stimulation by Cd2+ but rather inhibition at high Cd2+ concentrations
A910G
-
mutants more sensitive to Cd2+ than wild type strain, higher activity for glutathione S-conjugated leukotriene C4
D777N/A1003V
-
intragenic suppressor of mutation D777N
D777N/A1021T
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/A1021V
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/F565L
-
intragenic suppressor of mutation D777N, involved in determination of substrate specificity
D777N/G1207D
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/G1207S
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/N1027D
-
intragenic suppressor of mutation D777N
D777N/Q1107R
-
intragenic suppressor of mutation D777N, involved in determination of substrate specificity
D777N/R1415G
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/S1212L
-
intragenic suppressor of mutation D777N, functionally important residue
D777N/S674L
-
intragenic suppressor of mutation D777N
D777N/V543I
-
intragenic suppressor of mutation D777N, involved in determination of substrate specificity
D777N/W1225C
-
intragenic suppressor of mutation D777N, involved in determination of substrate specificity
D821G
-
mutants with Ycf1p in vivo activity as wild type strain
E709Q
-
mutants with Ycf1p in vivo activity as wild type strain
E927K
-
mutants exhibit a Cd2+ hypersensitive phenotype
G1306E
-
mutants exhibit a Cd2+ hypersensitive phenotype, abolished transport activity for glutathione S-conjugated leukotriene C4
G1311R
-
mutants exhibit a Cd2+ hypersensitive phenotype, abolished transport activity for glutathione S-conjugated leukotriene C4
G1413D
-
mutants exhibit a Cd2+ hypersensitive phenotype
G663V
-
mutants exhibit a Cd2+ hypersensitive phenotype, abolished transport activity for glutathione S-conjugated leukotriene C4
G756S
-
substitution mutation, unable to complement the cadmium hypersensitivity of the introduced strain
G835R
-
mutants more sensitive to Cd2+ than wild type strain, partially reduced transport activity for glutathione S-conjugated leukotriene C4
I711S
-
mutants exhibit a Cd2+ hypersensitive phenotype
I840P
-
mutants more sensitive to Cd2+ than wild type strain, partially reduced transport activity for glutathione S-conjugated leukotriene C4
L817S
-
mutants more sensitive to Cd2+ than wild type strain
L825T
-
mutants more sensitive to Cd2+ than wild type strain
L826S
-
mutants more sensitive to Cd2+ than wild type strain, partially reduced transport activity for glutathione S-conjugated leukotriene C4
N1366K
-
mutants exhibit a Cd2+ hypersensitive phenotype, partially reduced transport activity for glutathione S-conjugated leukotriene C4
Q1148P
-
mutants more sensitive to Cd2+ than wild type strain
R1143C
-
mutants more sensitive to Cd2+ than wild type strain, partially reduced transport activity for glutathione S-conjugated leukotriene C4
S251A
-
the site-directed mutation inhibits phosphorylation, the mutant exhibits increased resistance to cadmium in vivo and increased Ycf1p-dependent transport of [3H]estradiol-beta-17-glucuronide in vitro as compared with wild-type Ycf1p
S251E
-
the site-directed mutation mimics phosphorylation, activity is restored to the wild-type level for mutant Ycf1-S251E
S908A
-
replacement of Ser908 by alanine
S908A/T911A
-
the mutant shows a 50% diminished expression of Ycf1p compared to the wild-type enzyme
S908D
-
replacement of Ser908 by aspartate
S908E
-
replacement of Ser908 by glutamate
S908T
-
replacement of Ser908 by threonine
Y855L
-
mutants more sensitive to Cd2+ than wild type strain, higher activity for glutathione S-conjugated leukotriene C4
D777N
-
mutants exhibit a Cd2+ hypersensitive phenotype, partially reduced transport activity for glutathione S-conjugated leukotriene C4
D777N
-
strong defect in affinity for ATP and in transport velocity
G756D
-
mutants exhibit a Cd2+ hypersensitive phenotype, abolished transport activity for glutathione S-conjugated leukotriene C4
G756D
-
substitution mutation, unable to complement the cadmium hypersensitivity of the introduced strain
K669M
-
substitution mutation, unable to complement the cadmium hypersensitivity of the introduced strain
K669M
enzyme Ycf1p containing a mutation of the conserved Lys669 to Met in the Walker A box of the first nucleotide-binding domain (Ycf1pK669M) is unable to complement the fusion defect of DELTAycf1 vacuoles
additional information
Q0WLU3
despite sharing homology to the mitochondrial ABC1 of Saccharomyces cerevisiae, Arabidopsius thaliana OSA1 is not able to complement yeast strains deleted in the endogenous ABC1 gene. The atosa1-1 and atosa1-2 T-DNA insertion mutants are more affected than wild-type plants by Cd2+ and reveal an increased sensitivity toward oxidative stress caused by hydrogen peroxide and highlight. The mutants exhibit higher superoxide dismutase activities and differences in the expression of genes involved in the antioxidant pathway, phernotype, overview
additional information
heterologously expressed DmHMT-1 suppresses the Cd2+ hypersensitivity of Schizosaccharomyces pombe hmt-1 mutants and localizes to the vacuolar membrane but does not transport Cd-PCc, phenotype, overview
additional information
-
deletion mutant lacking metal-binding domain, domain is not required for Cd2+ binding for transport, stimulation by Cd2+ is similar for mutant and wild-type
additional information
-
deletion mutant lacking metal-binding domain, still acts as functional Cd2+-ATPase, probably interacts with metal transport site
additional information
construction of the full-length or truncated versions of OsHMA3 cDNAs, designated as OsHMA3-C1004, OsHMA3-C889, OsHMA3-C836, and OsMA3-C788, and transgenic expression in enzyme-deficient Arabidopsis thaliana Col-0 line, phenotypes, overview. Root lengths of the C899 and C836 lines are not significantly different from those of the C1004 (full length of OsHMA3) line, whereas they are significantly longer than for Arabidopsis thaliana wild-type line Col-0. The truncated OsHMA3-C788 is located on the vacuolar membrane, thereby losing its Cd transport function
additional information
-
construction of the full-length or truncated versions of OsHMA3 cDNAs, designated as OsHMA3-C1004, OsHMA3-C889, OsHMA3-C836, and OsMA3-C788, and transgenic expression in enzyme-deficient Arabidopsis thaliana Col-0 line, phenotypes, overview. Root lengths of the C899 and C836 lines are not significantly different from those of the C1004 (full length of OsHMA3) line, whereas they are significantly longer than for Arabidopsis thaliana wild-type line Col-0. The truncated OsHMA3-C788 is located on the vacuolar membrane, thereby losing its Cd transport function
additional information
-
deletion of L712 and F713, mutants exhibit a Cd2+ hypersensitive phenotype
additional information
-
deletion of the ycf1
additional information
-
deletion of F713, mutant protein not localized to the vacuole, no cadmium resistance
additional information
-
construction of the BY4741 haploid strain and null mutants. Fusion of the metal-responding degradation signal encompassing amino acids 250-350 to a stable protein demonstrates that it functions autonomously in a metal-responsive manner. Pca1 is not stabilized in a strain defective in endocytosis. Mutation of all seven cysteine residues to alanine alters the regulation of the enzyme expression, overview
additional information
-
deletion of the two kinase genes CKA1 and HAL5 increases Ycf1p function
additional information
-
increased accumulation of GSH in the ycf1 deletion strain during respiratory metabolism
additional information
-
construction of the BY4741 haploid strain and null mutants. Fusion of the metal-responding degradation signal encompassing amino acids 250-350 to a stable protein demonstrates that it functions autonomously in a metal-responsive manner. Pca1 is not stabilized in a strain defective in endocytosis. Mutation of all seven cysteine residues to alanine alters the regulation of the enzyme expression, overview
-
additional information
-
increased accumulation of GSH in the ycf1 deletion strain during respiratory metabolism
-
additional information
-
SpHMT-1-deficient cells are hypersensitive to Cd2+, but not to Hg2+ or As3+, heterologously expressed Drosophila melanogaster HMT-1 suppresses the Cd2+ hypersensitivity of Schizosaccharomyces pombe hmt-1 mutants and localizes to the vacuolar membrane but does not transport Cd-PCc, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Li, Z.S.; Szczypka, M.; Lu, Y.P.; Thiele ,D.J.; Rea, P.A.
The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump
J. Biol. Chem.
217
6509-6517
1996
Saccharomyces cerevisiae
-
brenda
Ren, X.Q.; Furukawa, T.; Chen, Z.S.; Okumura, H.; Aoki, S.; Sumizawa, T.; Tani, A.; Komatsu, M.; Mei, X.D.; Akiyama S.
Functional comparison between YCF1 and MRP1 expresses in Sf21 insect cells
Biochem. Biophys. Res. Commun.
270
608-615
2000
Saccharomyces cerevisiae
brenda
Petrovic, S.; Pascolo, L.; Gallo, R.; Cupelli, F.; Ostrow, J.D.; Goffeau, A.; Tiribelli, C.; Bruschi, C.V.
The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae
Yeast
16
561-571
2000
Saccharomyces cerevisiae
brenda
Jungwirth, H.; Wendler, F.; Platzer, B.; Bergler, H.; Hgenauer, G.
Diazaborine resistance in yeast involves the efflux pumps Ycf1p and Flr1p and is enhanced by a gain-of-function allele of gene YAP1
Eur. J. Biochem.
267
4809-4826
2000
Saccharomyces cerevisiae
brenda
Falcon-Perez, J.M.; Mazon, M.J.; Molano, J.; Eraso, P.
Functional domain analysis of the yeast ABC transporter Ycf1p by site-directed mutagenesis
J. Biol. Chem.
274
23584-23590
1999
Saccharomyces cerevisiae
brenda
Rebbeor, J.F.; Connolly, G.C.; Dumont, M.E.; Ballatori, N.
ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins
J. Biol. Chem.
273
33449-33454
1998
Saccharomyces cerevisiae
brenda
Wemmie, J.A.; Moye-Rowley, W.S.
Mutational analysis of the Saccharomyces cerevisiae ATP-binding cassette transporter protein Ycf1p
Mol. Microbiol.
25
683-694
1997
Saccharomyces cerevisiae
brenda
Wemmie, J.A.; Szczypka, M.S.; Thiele, D.J.; Moye-Rowley, W.S.
Cadmium tolerance mediated by the yeast AP-1 protein requires the presence of an ATP-binding cassette transporter-encoding gene, YCF1
J. Biol. Chem.
269
32592-32597
1994
Saccharomyces cerevisiae
brenda
Szczypka, M.S.; Wemmie, J.A.; Moye-Rowley, W.S.; Thiele, D.J.
A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein
J. Biol. Chem.
269
22853-22857
1994
Saccharomyces cerevisiae
brenda
Ortiz, D.F.; Kreppel, L.; Speiser, D.M.; Scheel, G.; McDonald, G.; Ow, D.W.
Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter
EMBO J.
11
3491-3499
1992
Schizosaccharomyces pombe
brenda
Bal, N.; Wu, C.C.; Catty, P.; Guillain, F.; Mintz, E.
Cd2+ and the N-terminal metal-binding domain protect the putative membranous CPC motif of the Cd2+-ATPase of Listeria monocytogenes
Biochem. J.
369
681-685
2003
Listeria monocytogenes
brenda
Bal, N.; Mintz, E.; Guillain, F.; Catty, P.
A possible regulatory role for the metal-binding domain of CadA, the Listeria monocytogenes Cd2+-ATPase
FEBS Lett.
506
249-252
2001
Listeria monocytogenes
brenda
Falcon-Perez, J.M.; Martinez-Burgos, M.; Molano, J.; Mazon, M.J.; Eraso, P.
Domain interactions in the yeast ATP binding cassette transporter Ycf1p: intragenic suppressor analysis of mutations in the nucleotide binding domains
J. Bacteriol.
183
4761-4770
2001
Saccharomyces cerevisiae
brenda
Eraso, P.; Martinez-Burgos, M.; Falcon-Perez, J.M.; Portillo, F.; Mazon, M.J.
Ycf1-dependent cadmium detoxification by yeast requires phosphorylation of residues Ser908 and Thr911
FEBS Lett.
577
322-326
2004
Saccharomyces cerevisiae
brenda
Koh, E.; Song, W.; Lee, Y.; Kim, K.H.; Kim, K.; Chung, N.; Lee, K.; Hong, S.; Lee, H.
Expression of yeast cadmium factor 1 (YCF1) confers salt tolerance to Arabidopsis thaliana
Plant Sci.
170
534-541
2006
Arabidopsis thaliana
brenda
Preveral, S.; Ansoborlo, E.; Mari, S.; Vavasseur, A.; Forestier, C.
Metal(loid)s and radionuclides cytotoxicity in Saccharomyces cerevisiae. Role of YCF1, glutathione and effect of buthionine sulfoximine
Biochimie
88
1651-1663
2006
Saccharomyces cerevisiae
brenda
Nagy, Z.; Montigny, C.; Leverrier, P.; Yeh, S.; Goffeau, A.; Garrigos, M.; Falson, P.
Role of the yeast ABC transporter Yor1p in cadmium detoxification
Biochimie
88
1665-1671
2006
Saccharomyces cerevisiae
brenda
Paumi, C.M.; Chuk, M.; Chevelev, I.; Stagljar, I.; Michaelis, S.
Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension
J. Biol. Chem.
283
27079-27088
2008
Saccharomyces cerevisiae
brenda
Adle, D.J.; Lee, J.
Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal
J. Biol. Chem.
283
31460-31468
2008
Saccharomyces cerevisiae
brenda
Sooksa-nguan, T.; Yakubov, B.; Kozlovskyy, V.I.; Barkume, C.M.; Howe, K.J.; Thannhauser, T.W.; Rutzke, M.A.; Hart, J.J.; Kochian, L.V.; Rea, P.A.; Vatamaniuk, O.K.
Drosophila ABC transporter, DmHMT-1, confers tolerance to cadmium. DmHMT-1 and its yeast homolog, SpHMT-1, are not essential for vacuolar phytochelatin sequestration
J. Biol. Chem.
284
354-362
2009
Drosophila melanogaster (Q9VF20), Schizosaccharomyces pombe
brenda
Jasinski, M.; Sudre, D.; Schansker, G.; Schellenberg, M.; Constant, S.; Martinoia, E.; Bovet, L.
AtOSA1, a member of the Abc1-like family, as a new factor in cadmium and oxidative stress response
Plant Physiol.
147
719-731
2008
Arabidopsis thaliana (Q0WLU3)
brenda
Mielniczki-Pereira, A.A.; Schuch, A.Z.; Bonatto, D.; Cavalcante, C.F.; Vaitsman, D.S.; Riger, C.J.; Eleutherio, E.C.; Henriques, J.A.
The role of the yeast ATP-binding cassette Ycf1p in glutathione and cadmium ion homeostasis during respiratory metabolism
Toxicol. Lett.
180
21-27
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
brenda
Schwartz, M.S.; Benci, J.L.; Selote, D.S.; Sharma, A.K.; Chen, A.G.; Dang, H.; Fares, H.; Vatamaniuk, O.K.
Detoxification of multiple heavy metals by a half-molecule ABC transporter, HMT-1, and coelomocytes of Caenorhabditis elegans
PLoS ONE
5
e9564
2010
Caenorhabditis elegans, Caenorhabditis elegans (G5EFD4)
brenda
Adle, D.J.; Wei, W.; Smith, N.; Bies, J.J.; Lee, J.
Cadmium-mediated rescue from ER-associated degradation induces expression of its exporter
Proc. Natl. Acad. Sci. USA
106
10189-10194
2009
Saccharomyces cerevisiae
brenda
Kumagai, S.; Suzuki, T.; Tezuka, K.; Satoh-Nagasawa, N.; Takahashi, H.; Sakurai, K.; Watanabe, A.; Fujimura, T.; Akagi, H.
Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3
FEBS Lett.
588
789-794
2014
no activity in Arabidopsis thaliana ecotype Columbia, Oryza sativa (Q8H384), Oryza sativa
brenda
Sasser, T.L.; Lawrence, G.; Karunakaran, S.; Brown, C.; Fratti, R.A.
The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion
J. Biol. Chem.
288
18300-18310
2013
Saccharomyces cerevisiae (P39109), Saccharomyces cerevisiae
brenda
Wang, Y.; Wang, C.; Liu, Y.; Yu, K.; Zhou, Y.
GmHMA3 sequesters Cd to the root endoplasmic reticulum to limit translocation to the stems in soybean
Plant Sci.
270
23-29
2018
Glycine max
brenda
Smith, A.T.; Ross, M.O.; Hoffman, B.M.; Rosenzweig, A.C.
Metal selectivity of a Cd-, Co-, and Zn-transporting P1B-type ATPase
Biochemistry
56
85-95
2017
Cupriavidus metallidurans (Q1LAJ7)
brenda